数字农科院2.0

综纤维素酶水解玉米秸秆糖化特性及工艺优化

文献类型: 中文期刊

关键词: 纤维素酶;;水解;;响应面;;秸秆

期刊名称:农业工程学报/Transactions of the Chinese Society of Agricultural Engineering

ISSN: 1002-6819

年卷期: 2025 年

页码:

收录情况: EI(2025版) ; ; 北大核心(2023版) ; ; CSCD(2025-2026年度) ; ; 科技核心(2024版) ; ; 农林核心(2024版)

摘要: 为突破高固含量酶解玉米秸秆综纤维素过程中的传质限制与产物抑制瓶颈,本研究将纤维素酶、纤维二糖酶和木聚糖酶复配,先通过单因素实验确定关键参数区间,再采用Box-Bchnken设计结合响应面法优化底物固含量、酶添加量、温度及停留时间。结果表明:在含固量20%、酶添加量3倍、48.21℃、72h的最优条件下,还原糖浓度达108.97gL,产率94.49%,较初始工艺提高21.3%:表征结果证实酶解后底物表面孔原率增加,纤维素结晶度由13.91%降至9.43%,木质素一半纤维素屏障被破坏。该工艺提升了高固体系酶解效率,为木质纤维素生物炼制的工业化提供了技术支撑。

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