数字农科院2.0

麦长管蚜9个miRNA在不同发育阶段的表达

文献类型： 中文期刊

作者： 张方梅;闫艺;程登发;朱勋;张云慧;李祥瑞

作者机构：

关键词： 麦长管蚜; miRNA; U6; 内参基因; 翅型分化; 表达谱

期刊名称：昆虫学报

ISSN： 0454-6296

年卷期： 2022 年 01 期

页码：

收录情况： 北大核心（2020版） ; CSCD（2021-2022年度） ; 科技核心（2021版） ; 农林核心（2020版）

摘要： [目的]探讨麦长管蚜Sitobion avenae 9个微小RNAs（miRNA）在两翅型间不同发育阶段的表达模式,揭示其在蚜虫翅型分化中发挥作用的具体时期。[方法]RT-PCR克隆麦长管蚜内参基因U6的cDNA序列和9个miRNA,并利用qRT-PCR方法检测9个miRNA在有翅和无翅麦长管蚜不同发育阶段（伪胚胎、1-4龄若蚜和成蚜）的表达。[结果]麦长管蚜U6的cDNA全长为92 bp,与其他昆虫的U6 cDNA序列一致性达80%以上。qRT-PCR结果表明,大部分miRNA在伪胚胎期及若蚜期的相对表达量均较低,在成蚜期相对表达量较高。6个miRNA（miR-277,miR-9a,miR-7,Let-7,miR-1和miR-315）在有翅成蚜中的相对表达量高于无翅成蚜中的。miR-277和miR-9a在有翅成蚜期的相对表达量较高。除miR-315外,miR-277,miR-9a,miR-7,Let-7和miR-1在成蚜期的相对表达量在两翅型间均呈显著差异,且miR-9a在伪胚胎期和miR-315在3龄若蚜时期相对表达量在两翅型间也差异显著。其他3个miRNA（miR-8,PC-3p-2743844和PC-5p-11319015）在无翅成蚜中的相对表达量高于有翅成蚜中的。其中miR-8和PC-3p-2743844在有翅成蚜和无翅成蚜期表达量显著高于在其他发育阶段,而PC-5p-11319015在无翅蚜3龄若蚜期显著高于其他发育阶段。miR-8在成蚜期的相对表达量在两翅型间差异显著,PC-3p-2743844在伪胚胎和成蚜期的相对表达量在两翅型间差异显著。PC-5p-11319015在3龄若蚜时期的相对表达量在两翅型间差异极显著。[结论]麦长管蚜内参基因U6与其他昆虫的U6 cDNA序列具有高度保守性。表达谱分析显示,9个miRNA可能在伪胚胎或成虫阶段在麦长管蚜翅型分化中起调控作用。

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