G-四链体序列筛选及G-四链体等温信号扩增体系的构建
文献类型: 中文期刊
作者: 何杉;盛磊;高进;苗朝华;刘卫晓;宛煜嵩;金芜军
作者机构:
关键词: G-四链体;DNAzyme;等温信号扩增
期刊名称:分子植物育种
ISSN: 1672-416X
年卷期: 2022 年
页码:
收录情况: 北大核心(2020版) ; 科技核心(2021版) ; 农林核心(2020版)
摘要: G-四链体序列可与Hemin结合形成具有过氧化物酶活性的DNAzyme,被广泛应用于生物传感器的构建中。G-四链体的过氧化物酶活性高低对生物传感器构建至关重要。本研究利用圆二色谱比较分析了生物传感器常用G-四链体序列的空间构型,筛选出过氧化物酶活性高的G-四链体序列,并比较了G-四链体中鸟苷酸聚集单元数、K+及Hemin对其过氧化物酶活性的影响。本研究应用高过氧化物酶活性的G-四链体作为可视检测信号核酸分子设计了一种可用于核酸检测的G-四链体等温信号扩增体系,不同于通常核酸检测方法直接对目标序列进行扩增,该体系在等温条件下,通过剪切与置换进一步大量扩增作为信号分子的G-四链体序列,致使整个核酸检测过程更为简便,为生物传感器构建、新型核酸检测技术研发提供了新的思路和技术支持。
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