数字农科院2.0

用DGGE和Real-Time PCR对低温沼气池中产甲烷古菌群落的研究

文献类型： 中文期刊

作者： 王彦伟;徐凤花;阮志勇;宋金龙;王庆;赵斌;ZHAO Bin

作者机构：

关键词： 沼泥;产甲烷古菌;DGGE;Real-Time PCR

期刊名称：中国沼气

ISSN： 1000-1166

年卷期： 2012 年 30 卷 01 期

页码： 8-12

摘要： 利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(A1)发酵后期成为优势群落,甲烷鬃毛菌属(Methanosaeta)在山东发酵前期(B1),后期样品(B2)及安徽发酵前期样品(C1)中占有绝对优势。产甲烷古菌的数量介于104～105个.mL-1之间,其中黑龙江样品中产甲烷菌数量最多,而安徽样品中产甲烷菌数量最少。

分类号： X172

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