数字农科院2.0

一种无需DEPC提取高质量棉花总RNA的方法

文献类型: 中文期刊

作者: 刘冬梅;丁锦平;李成伟;李付广

作者机构:

关键词: 棉花;DEPC;改良CTAB法;RNA;提取方法

期刊名称:江西农业学报

ISSN: 1001-8581

年卷期: 2009 年 21 卷 07 期

页码: 15-17

摘要: DEPC做为RNAnase抑制剂常用于RNA的提取,但其本身具有毒性,容易对操作者产生伤害并易造成环境污染。该实验以改良CTAB法为基础,提高β-巯基乙醇的浓度到10%,无需DEPC就能提取高质量的RNA。分别以不同陆地棉为材料提取了棉花的总RNA,测得OD260/OD280在1.7~2.1之间,且大部分样品都能清晰看到3条RNA条带,将此RNA反转录获得的cDNA为模板克隆到了棉花ATP合成酶β亚基基因,说明此方法提取的棉花总RNA既能满足分子生物学实验要求又大大地减少了毒害作用。

分类号: S562

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