数字农科院2.0

内蒙古黄瓜靶斑病菌的鉴定及药剂敏感性测定

文献类型： 中文期刊

作者： 杨立辉;陈鹏宇;王友贤;刘宝玉;白庆荣;赵廷昌

作者机构：

关键词： 黄瓜靶斑病;Corynespora cassiicola;病原鉴定;药剂筛选

期刊名称：中国瓜菜

ISSN： 1673-2871

年卷期： 2022 年

页码：

收录情况： 北大核心（2020版） ; ; 农林核心（2020版）

摘要： 笔者对来自内蒙古自治区赤峰市宁城县五化镇山头村的黄瓜叶斑病标本进行组织分离，纯化得到20株分离物，经柯氏验证均为致病菌。结合病菌形态学特征和分子生物学相关序列分析，确定该病原菌为多主棒孢菌[Corynespora cassiicola（Berk ＆ Curt）Wei]。采用菌丝生长速率法和孢子萌发法测定C. cassiicola对26种药剂的敏感性。结果表明，代表菌株HGBB-Ⅲ菌丝生长对25%咪鲜胺EC、40%肟菌·咪鲜胺EW、40%苯醚甲环唑SC和10%苯醚甲环唑WG的敏感性较高，EC50＜1 mg·L-1；对430 g·L-1戊唑醇SC、50%腐霉利WP、50%福美双WP、50%异菌脲WP、75%肟菌·戊唑醇WG、40%唑醚·戊唑醇SC、32.5%苯甲·嘧菌酯SC、30%肟菌·戊唑醇SC的敏感性略低，1 mg·L-1＜EC50＜10 mg·L-1。病菌的孢子萌发对50%福美双WP的敏感性最高，EC50＜1 mg·L-1，对42.4%唑醚·氟酰胺SC、200 g·L-1氟酰羟·苯甲唑SC、40%唑醚·戊唑醇SC、12%苯甲·氟酰胺SC的敏感性较低，10 mg·L-1＜EC50＜100 mg·L-1。上述药剂中，50%福美双WP对病原菌菌丝生长和孢子萌发的抑制效果均较好，可以作为防治过程中首选药剂。

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