数字农科院2.0

甜菜SRAP扩增产物两种不同检测方法的比较

文献类型： 中文期刊

作者： 吴则东;王华忠;韩英

作者机构：

关键词： 甜菜;SRAP;电泳方法;银染方法

期刊名称：中国糖料

ISSN： 1007-2624

年卷期： 2010 年 02 期

页码： 9-11

摘要： 为了研究不同电泳及染色方法对甜菜SRAP扩增产物的影响,以6个甜菜二倍体品系为材料,比较变性聚丙烯酰胺凝胶电泳与非变性聚丙烯酰胺凝胶电泳对SRAP-PCR扩增产物用不同银染方法的检测效果进行了比较分析。结果显示:非变性聚丙烯酰胺凝胶电泳与变性聚丙烯酰胺凝胶电泳的分辨率差异不大,但非变性聚丙烯酰胺凝胶电泳具有灌胶简单易学,显色时间短,节省药品等特点,可以作为今后甜菜SRAP电泳及显色的首选方法。

分类号： S566.301

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