水稻分蘖调控基因HTD3 的克隆与功能研究
文献类型: 中文期刊
作者: 吴先美;;李三峰;;胡萍;;何瑞;;焦然;;毛一剑;;鲁草林;;胡娟;;林晗;;吴荣梁;;朱旭东;;饶玉春;;王跃星
作者机构:
关键词: 水稻;;多分蘖;;图位克隆;;生物学功能
期刊名称:中国水稻科学
ISSN: 1001-7216
年卷期: 2021 年
页码:
收录情况: 北大核心(2020版) ; CSCD(2020-2021年度) ; 农林核心(2020版) ; 科技核心(2020版)
摘要: 【目的】克隆水稻分蘖相关基因,为构建理想株型水稻,提高粮食产量提供理论基础及有利基因资源。【方法】在常规大田种植条件下分别比较突变体htd3(high-tillering dwarf 3)与野生型在幼苗期、抽穗期和成熟期表型及主要农艺性状差异,利用图位克隆方法克隆候选基因,利用荧光定量PCR 方法分析HTD3 及独脚金内酯和脱落酸相关基因的表达水平,测序比对分析HTD3 在147 份种质资源中的自然变异情况。【结果】与野生型相比,突变体htd3 的分蘖芽生长较快,分蘖数和有效穗数显著增多,株高、一次枝梗数和每穗粒数显著降低,结实率和千粒重没有显著变化。遗传分析表明,htd3 多分蘖的性状受一对隐性核基因控制。图位克隆将HTD3 基因定位在第12 染色体CM8 和CM10 之间约63.5 kb 的物理区间内,互补转基因实验证明该区间内LOC_Os12g21710为控制突变体多分蘖表型的基因。HTD3 在野生型和突变体中呈组成型表达,该基因突变会引起部分独脚金内酯和脱落酸相关基因的表达水平上调。水稻品种中HTD3 编码区G2674A 自然变异使得分蘖数显著增多。【结论】HTD3 是最近报道的T20/MIT1 基因的一个新的等位基因,HTD3 突变导致水稻出现分蘖适度增加,株高略矮的表型,在培育理想株型水稻和高产育种上具有较大的应用潜力。
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