数字农科院2.0

川纹笛鲷消化道优势菌群PCR-DGGE指纹图谱比较分析

文献类型： 中文期刊

作者： 周志刚;石鹏君;姚斌;何夙旭;苏永全

作者机构：

关键词： 川纹笛鲷(Lutjanus sebae);16S rDNA;梯度凝胶电泳;指纹图谱

期刊名称：海洋科学

ISSN： 1000-3096

年卷期： 2008 年 32 卷 11 期

页码： 11-14

收录情况： 北大核心 ; CSCD

摘要： 采用免培养的16S rDNA梯度凝胶电泳技术(DGGE)对集约化海水网箱养殖川纹笛鲷(Lutjanus sebae)的消化道胃壁、胃内容物、肠壁、肠内容物优势菌群结构进行了比较分析。研究结果显示,川纹笛鲷消化道存在着丰富多样的细菌群落,对DGGE指纹图谱聚类分析表明菌群组成相似度高于55%,其中胃内容物及胃壁细菌组成相似度最高(90%),这可能与摄食饵料在消化道推移有关;而胃壁与肠壁相似度相对最差,可能反应了由于生理环境不同引起的宿主差异性。通过建立川纹笛鲷消化道16SrDNA-DGGE指纹图谱及比较分析,为澄清川纹笛鲷消化道微生物区系奠定了基础。

分类号： S917.1

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