数字农科院2.0

药用牡丹典型病害高效防治药剂筛选及残留特征分析

文献类型： 中文期刊

关键词： 牡丹;;红斑病;;根结线虫病;;QuEChERS 技术;;残留分析

期刊名称：农药

ISSN： 1006-0413

年卷期： 2025 年

页码：

收录情况： 北大核心（2023版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： [目的]针对牡丹红斑病和根结线虫病高效防治药剂较少的问题，开展2 种病害高效防治药剂的筛选，并阐明其在牡丹上的残留特征。[方法]通过室内生物活性测试和大田防效试验，分别研究不同药剂对牡丹红斑病和根结线虫病的防治效果。建立基于QuEChERS 样品前处理技术和超高效液相色谱-串联质谱(UPLC-MS/MS)检测牡丹中不同药剂残留量的方法，并对方法的准确度和精密度进行评价。通过田间残留试验，明确各药剂在药用牡丹中的残留特征。[结果]吡唑醚菌酯、苯醚甲环唑和嘧菌酯对牡丹红斑病病原菌的抑制效果较好，EC50值分别为1.279、1.374、0.909 mg/L。三氟吡啶胺、氟吡菌酰胺对牡丹根结线虫的抑制效果较好，LC50 值分别为0.292、0.724 mg/L。进一步的大田防效结果表明，25% 吡唑醚菌酯悬浮剂、10% 苯醚甲环唑悬浮剂、30% 嘧菌酯悬浮剂和450 g/L 三氟吡啶胺悬浮剂、400 g/L 氟吡菌酰胺悬浮剂对2 种病害的防治效果较好，田间防效均大于80%。牡丹样品中残留的吡唑醚菌酯、苯醚甲环唑、氟吡菌酰胺、三氟吡啶胺经乙腈提取，25 mg PSA 净化，UPLC-MS/MS检测。在3 个添加水平下回收率为82.7%~95.1%，相对标准偏差(RSD)为1.4%~6.5%，线性相关系数(r)均>0.99。在全国牡丹主产区进行的1年4地残留试验结果表明，4种药剂在牡丹中的最终残留量中值(STMR)均<0.010 mg/kg，残留水平较低。[结论]为牡丹红斑病和根结线虫病的防治筛选出高效安全药剂，对保障牡丹产业的可持续发展具有重要意义。

分类号：

  • 相关文献

[1]4套防治罗汉果根结线虫病用药方案的实施效果. 李芮,,祈艳华,,陈艳露,,汪斌,,李世东,,卢晓红,, 孙漫红,,罗明,,郭荣君. 2025

[2]The Nicotiana benthamiana microtubule-associated E3 ubiquitin ligase degrades a host immune regulator and a viral effector to enhance antiviral defense. 王坤,,,,傅帅,,,,谭羽翀,,,,吴良,,,,王亚琴,,,,周雪平. 2025

[3]Fine-tuning of the CRWN2-NTL9 module to repress PR1 expression by a viral effector during geminivirus infection. 苏晨露,,,,王亚琴,,,,李方方,,,,梅玉振,,,,周雪平. 2025

[4]A key heavy metal-binding protein orchestrates plant resistance against a geminivirus. 刘慧,,,,胡涛,,,,李方方,,,,王亚琴,,,,梅玉振,,,,周雪平. 2025

[5]Divergence in global plant carbon use efficiency across data-driven estimates, satellite product, and process-oriented models. Lei He,,,,Josep Peñuelas,,,,Jingfeng Xiao,,,,Xiaolu Tang,,,,Yue He,,,,Menglin Si,,,,Zhao-Liang Li. 2025

[6]Reconstruction of cloudy land surface temperature by combining surface energy balance theory and solar-cloud-satellite geometry. Wenhui Du,,,,Zhao-Liang Li,,,,Zhihao Qin,,,,Jinlong Fan,,,,Xiangyang Liu,,,,Chunliang Zhao,,,,Kun Cao. 2025

[7]Contrasting temporal dynamics of land surface temperature response to different types of forest loss. Jing Li,,,,Zhao-Liang Li,,,,Xiangyang Liu,,,,Yitao Li,,,,Meng Liu,,,,Nanshan You,,,,Hua Wu,,,,Lei He,,,,Menglin Si,,,,Ronglin Tang,,,,Chenghu Zhou,,,,Wei Zhao,,,,Si-Bo Duan,,,,Pei Leng,,,,Wenqi Liu,,,,Enyu Zhao,,,,Bo-Hui Tang,,,,Zhenong Jin. 2025

[8]Key genes and mechanisms underlying natural variation of silique length in oilseed rape (Brassica napus L.) germplasm. Quaid Hussain, Jiepeng Zhan, Huabing Liang, Xinfa Wang, Guihua Liu, Jiaqin Shi, Hanzhong Wang. 2021

[9]Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops. Zaman, Qamar U.,Raza, Ali,Lozano-Juste, Jorge,Chao, Li,Jones, Michael G. K.,Wang, Hua-Feng,Varshney, Rajeev K.. 2024

[10]上部烟叶灰分及其钙、镁、硫、磷含量与烟叶品质的关系及近红外模型研究. 付秋娟,,郝贤伟,,杜咏梅,,毕一鸣,,孙婷婷. 2025

[11]Evaluation of different clean heat supply modes based on crop straws in the rural areas of Northern China. 霍丽丽,,姚宗路,,贾吉秀,,赵立欣,,丛宏斌,,孟海波,,袁艳文. 2020

[12]Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311. 乔卫华,,齐兰,,程志军,,苏龙,,李静,,孙妍,,任军芳,,郑晓明,,杨庆文. 2016

[13]OsCOL16, encoding a CONSTANS-like protein, represses flowering by up-regulating Ghd7, expression in rice. 吴玮勋,,郑晓明,,陈代波,,张迎新,,马薇薇,,张欢,,孙连平,,杨峥赋,,赵春德,,詹晓德,,沈喜红,,于萍,,付亚平,,朱珊珊,,曹立永,,程式花. 2017

[14]An egg parasitoid assesses host egg quality from afar using oviposition-induced plant volatiles. Tian, Zhiqiang,Wang, Yuanyuan,Sun, Tao,Hu, Xiaoyun,Hao, Wanting,Zhao, Te,Wang, Yanan,Zhang, Lei,Jiang, Xingfu,Turlings, Ted C. J.,Li, Yunhe. 2025

[15]Regulation of plant immunity through histone H3 β-hydroxybutyrylation-mediated transcriptional control. 徐秋涛,,陈正庭,,王瑞,,,,马瑄,,,,段宇航,,,,海杜,,,,陈静,,,,程仲毅,,,,郑路,,,,黄俊斌,,,,张积森,,,,赵毓,,,,陈晓洋. 2025

[16]A glutamyl–tRNA reductase and its binding protein promote transitory starch biosynthesis and enhance grain quality and yield in rice. 段影青,李晓雪,马刘洋,焦桂爱,操瑞节,董楠楠,李欣伟,王梓航,王平,唐绍清,魏祥进,胡培松. 2025

[17]Epi-transcriptome analysis reveals the lack of N6-methyladenosine modification in two RNA viruses infecting watermelon. 潘福安,,,,荆嘉贤,,,, Yanhong Qiu,,,,Maoying Li,,,,周雪平,,,,李少芳. 2025

[18]黄曲霉毒素阻控与花生超级结瘤耦合效应研究初报. 周扬,,岳晓凤,,唐晓倩,,闫洪林,,张奇,,李培武. 2021

[19]盐碱地麦后复种绿肥与氮肥减施对小麦产量、环境 足迹及经济效益的协同效应. 张梦璇,,常芳弟,,魏禾雅,,李晓红,,伍林美,,张宏媛. 2025

[20]Mobile genetic elements affect the dissemination of antibiotic resistance genes (ARGs) of clinical importance in the environment. Ye Li ,,,,Ruilin Li ,,,,Jie Hou ,,,, Xuan Sun ,,,, Yajun Wang,,,, Linyun Li ,,,,Fengxia Yang ,,,,Yanpo Yao ,,,, Yi An. 2023

作者其他论文 更多>>