数字农科院2.0

中国南瓜曲叶病毒侵染新疆吐鲁番西葫芦的分子检测

文献类型: 中文期刊

作者: 储天然;廖锴;罗文芳;何伟;许建军;王少骅;石延霞;谢学文;李磊;范腾飞;李宝聚;柴阿丽

作者机构:

关键词: 中国南瓜曲叶病毒;分子鉴定;SLCCNV;PCR

期刊名称:中国蔬菜

ISSN: 1000-6346

年卷期: 2025 年

页码:

收录情况: 北大核心(2023版) ; ; 科技核心(2024版) ; ; 农林核心(2024版)

摘要: 2 023年在新疆吐鲁番部分西葫芦植株上发现叶片卷曲、扭曲、褪绿、黄化等疑似病毒病的症状,为精确鉴定侵染新疆吐鲁番地区西葫芦的病毒种类,利用4对DNA双组分双生病毒引物和10对RNA病毒引物对采集样品进行检测,并对含有目标条带的扩增产物进行测序,序列提交至NCBI数据库并进行BLAST比对后进行聚类分析。结果表明,两组中国南瓜曲叶病毒(squash leaf curl China virus,SLCCNV)特异性引物分别扩增出了742 bp和539 bp的特异性目标片段。BLAST比对显示采自新疆吐鲁番地区的病毒分离物与SLCCNV聚在同一分支上,亲缘关系较近,与数据库中其他病毒分离物存在相对较远的遗传距离,说明新疆吐鲁番地区的西葫芦植株被中国南瓜曲叶病毒所侵染。

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