数字农科院2.0

弓形虫上海株SAG2基因克隆和序列分析

文献类型: 中文期刊

作者: 陈志强;程天印;王权;陈永军

作者机构:

关键词: 弓形虫;SAG2;PCR

期刊名称:中国兽医寄生虫病

ISSN: 1005-0868

年卷期: 2007 年 15 卷 02 期

页码: 12-15

摘要: 目的:对弓形虫上海株SAG2基因进行克隆与鉴定。方法:采用PCR方法从弓形虫上海株总DNA中扩增到SAG2基因,与pGEM-T-easy vector连接,并进行DNA测序分析。结果:用DNAStar软件对扩增出的片断与GenBank上的7个虫株相应的序列进行同源性比较,不同宿主和不同区域上的弓形虫SAG2基因序列差异很小。结论:用基因重组技术获得的SAG2抗原,作为候选疫苗和诊断抗原,有着广阔的发展前景。

分类号: S852.7

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