甜菜EST-SSR引物的快速筛选
文献类型: 中文期刊
作者: 吴则东;邹兰兰;刘乃新;马龙彪;倪洪涛
作者机构:
关键词: 甜菜;EST-SSR;筛选;退火温度
期刊名称:中国农学通报
ISSN: 1000-6850
年卷期: 2016 年 32 卷 36 期
页码: 104-108
摘要: 为了快速从甜菜EST-SSR引物中筛选出适合甜菜分子生物学的引物,利用12个不同的甜菜品种对80对甜菜EST-SSR引物进行扩增,同时设置12个不同的退火温度,利用8%的非变性聚丙烯酰胺凝胶对PCR产物进行分离。结果表明:80对EST-SSR引物虽然均能够扩增出产物,但绝大部分引物扩增的条带由于没有多态性或者条带难以辨别而不能够使用,只有13对引物扩增出了清晰、易于识别的条带,有4对引物多态性较高,其余9对引物多态性均较低,而梯度退火温度则表明,退火温度对EST-SSR引物影响不大,只有个别引物会在较低退火温度条件下产生非特异性的条带,因此将所有引物的退火温度均设定为60℃。利用不同品种以及梯度退火温度可以实现EST-SSR引物的快速筛选,但要想筛选出多态性高的引物还需要对更多的EST-SSR引物进行筛选。
分类号: S566.3
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