新疆野杏桃褪绿叶斑病毒和梅相关黄症病毒的鉴定
文献类型: 中文期刊
作者: 米美璇;李世访;战斌慧;张志想;麦合木提江·米吉提
作者机构:
期刊名称:植物病理学报
ISSN: 0412-0914
年卷期: 2023 年
页码:
收录情况: 北大核心(2020版) ; ; CSCD(2023-2024年度) ; ; 科技核心(2023版) ; ; 农林核心(2020版)
摘要: Xinjiang wild apricot(Prunus armeniaca) has strong resistance to drought and cold. It is an important genetic resource for breeding. In 2021, twenty leaf samples of Xinjiang wild apricot were collected in the wild forests of Tianshan Mountain in Yili, Xinjiang. Using the high-throughput sequencing and RT-PCR, peach chlorotic leaf spot virus(PCLSV) and mume-associated luteovirus(Ma LV) were identified. Whole genomes of these two viruses were obtained by RT-PCR and RACE.After removing poly(A), the genome of PCLSV from Prunus armeniaca(PCLSV-YX) contains 7 462 nucleotides(nts) with three overlapping open reading frames(ORFs). The genome of MaLV from Prunus armeniaca(MaLV-YX) contains 5 753 nts, and has six ORFs. Phylogenetic analysis showed that PCLSV-YX was closely related to isolates of ‘PR19-2’ and ‘TaTao’. MaLV-YX was closely related to cherry-associated luteovirus(ChALV), Prunus mahaleb-associated luteovirus(PmALV), peach-associated luteovirus(PaLV) and peach-associated luteovirus 2(PaLV 2).
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