数字农科院2.0

水稻抗白叶枯病基因Xa23的PCR分子标记定位及铺助选择

文献类型： 中文期刊

作者： 潘海军;王春连;赵开军;章琦;樊颖论;周少川;朱立煌

作者机构：

关键词： Xa23;水稻白叶枯病;SSR;RAPD;MAS

期刊名称：作物学报

ISSN： 0496-3490

年卷期： 2003 年 29 卷 4 期

页码： 501-507

收录情况： CSCD

摘要： 摘要用Xa23的近等基因系CBB23及其感病轮回亲本JG30构建了包含2562个单株的F个感病单株，找到2个新的与Xa23基因连锁的SSR标记RM 187和RM206，它们与Xa23之间的遗传图距分别为7.1 cM和1.9 cm。通过筛选1200个RAPD引物，获得2个与Xa23基因连锁的RAPD标记RpdH5和RpdS1184，与Xa23之间的传图距分别为7.0 cM和7.6 cM。利用丰富占／/CBB23,绿油占／/CBB23两个实际育种F_2群体，结合抗病性人工接种鉴测算了RpdS 1184 , RM206和RpdH5用于分子标记辅助选择（MAS )的可能性。结果表明，对于丰富23群体，RpdH5和Rp-dS1184的MAS准确率分别为91.0％和87.3%，如果同时使用这两个标记，可使MAS的准确率达99%。对于绿油23群体，RpdH5,RpdS1184和RM206的MAS准确率分别为77.1%,81.1％和80.8%。同时使用RpdH5, RpdS1184标记的MAS准确率为90.3%；同时使用RpdH5,RM206的准确率为91.3%；同时使用RpdH5, RM206 , RpdS1184标记的准确率为90.8%.

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