数字农科院2.0

cDNA-AFLP和cDNA-SRAP技术在植物基因差异表达上的应用及其分析比较

文献类型: 中文期刊

作者: 李杨瑞;杨柳;王爱勤;杨丽涛;熊发前;甘崇昆

作者机构:

关键词: cDNA-AFLP;cDNA-SRAP;分子标记;差异表达

期刊名称:生物技术通报

ISSN: 1002-5464

年卷期: 2009 年 11 期

页码: 52-55

收录情况: 北大核心 ; CSCD

摘要: 对cDNA-AFLP和cDNA-SRAP技术在植物上的应用研究进行了介绍,指出cDNA-AFLP和cDNA-SRAP技术已逐渐成为基因表达研究的重要工具,是构建转录图谱、定位目标性状位点、研究差异基因表达等研究的主要手段,具有广泛广阔的应用前景。但是cDNA-AFLP和cDNA-SRAP技术均具有一定的缺限,主要表现在获得的差异片段还不够全面丰富。cD-NA-AFLP可以扩增到扩增较多的带,但试验程序比较繁琐、昂贵而且耗时较长、操作较困难;而cDNA-SRAP相对比较简便、便宜而耗时较短,较易操作。因而将cDNA-AFLP与cDNA-SRAP技术结合起来,可望得到更全面丰富的差异片段,从而在植物基因差异表达、新基因发现、抗逆性分子机理研究等方面发挥更大的作用。

分类号: Q943.2

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