金鸡河流域不同土地类型典型耐药菌和耐药基因的分布特征
文献类型: 中文期刊
关键词: 抗生素耐药菌(ARB);;抗生素耐药基因(ARGs);;土地利用类型;;微生物群落;;菜地
期刊名称:土壤学报
ISSN: 0564-3929
年卷期: 2025 年
页码:
收录情况: 北大核心(2023版) ; ; CSCD(2025-2026年度) ; ; 科技核心(2024版) ; ; 农林核心(2024版)
摘要: 为系统探究不同土地利用类型对土壤中抗生素耐药菌(ARB)及耐药基因(ARGs)分布的影响,采集北京市金鸡河流域 6 种典型土地利用类型(菜地、麦地、花圃、果园、苗圃和养殖场)土壤样品,对其总可培养细菌、金霉素耐药菌、磺胺甲噁唑耐药菌和代表性 ARGs 的丰度进行测定,并结合高通量测序技术分析典型土壤微生物群落结构特征。结果表明,金鸡河流域张镇和龙湾屯镇所受抗性污染程度最高,菜地土壤中 ARB、ARGs 及 intI1 的丰度显著高于其他土地利用类型(P<0.05);拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、髌骨细菌门(Saccharibacteria)和异常球菌-栖热菌门(Deinococcus-Thermus)是土壤中的优势菌门;溶杆菌(Lysobacter)和德沃斯氏菌(Devosia)是 ARGs 的主要宿主菌,与 sul1、sul2、tetG、tetX 及 intI1 均呈显著正相关(P<0.05)。金鸡河流域菜地土壤的耐药水平显著高于其他土地利用类型,应注重有机肥的优化施用以降低土壤微生物耐药性和生态健康的潜在风险。
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