数字农科院2.0

用DGGE和Real-TimePCR对低温沼气池中产甲烷古菌群落的研究

文献类型： 中文期刊

作者： 王彦伟;徐凤花;阮志勇;宋金龙;王庆;赵斌

作者机构：

关键词： 沼泥;产甲烷古菌;DGGE;Real-TimePCR

期刊名称：中国沼气

ISSN：

年卷期： 2012 年 01 期

页码： 8-12

收录情况： CSCD

摘要： 利用DGGE及Real-TimePCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-TimePCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(...

分类号：

  • 相关文献

[1]用DGGE和Real-Time PCR对低温沼气池中产甲烷古菌群落的研究. 王彦伟,徐凤花,阮志勇,宋金龙,王庆,赵斌,ZHAO Bin. 2012

[2]沼泥及其富集物中微生物多样性和区系变化的分析. 宋金龙,阮志勇,胡国全,姜瑞波,刘小飞,徐凤花. 2010

[3]浓香型白酒不同窖泥的微生物群落特征分析. 施思,王海英,张文学,邓宇,赖登燡,范鏖. 2011

[4]分子生物学技术在产甲烷古菌研究中的应用. 王刘阳,尹小波,胡国全. 2008

[5]瘤胃古菌C簇研究进展. 魏莎,梁贤威,梁辛,李孟伟,郭艳霞,杨承剑. 2017

[6]产甲烷古菌研究进展. 承磊,郑珍珍,王聪,张辉. 2016

[7]改进TAIL-PCR方法克隆产甲烷古菌的mcr基因簇. 朴玉华,徐彦胜,胡国全. 2013

[8]稻秸添加对两种水稻土产甲烷古菌及细菌的影响. 保琼莉,王凤花,保万魁,黄益宗. 2019

[9]反刍动物瘤胃甲烷生成相关研究进展. 王坤,南雪梅,熊本海,蒋林树. 2020

[10]绿肥稻田土壤甲烷减排材料筛选及减排机制研究. 孙晋鑫,周国朋,马正波,梁婷,常单娜,刘佳,刘晗,曹卫东. 2024

[11]绿肥稻田土壤甲烷减排材料筛选及减排机制研究. 孙晋鑫,周国朋,马正波,梁婷,常单娜,刘佳,刘晗,曹卫东. 2025

[12]Effect of Feeding Bacillus subtilis natto on Hindgut Fermentation and Microbiota of Holstein Dairy Cows. Song, D. J.,Kang, H. Y.,Wang, J. Q.,Peng, H.,Bu, D. P.. 2014

[13]Diversity and abundance of the rumen and fecal methanogens in Altay sheep native to Xinjiang and the influence of diversity on methane emissions. Liu, C.,Zhu, Z. P.,Dong, H. M.,Liu, Y. F.,Guo, T. J.. 2012

[14]Impact of Repeated Applications of Metalaxyl on Its Dissipation and Microbial Community in Soil. Wang, Fenghua,Zhu, Lusheng,Wang, Jun,Wang, Jinhua,Wang, Xiuguo.

[15]Urea- and nitrapyrin-affected N2O emission is coupled mainly with ammonia oxidizing bacteria growth in microcosms of three typical Chinese arable soils. Cui, Peiyuan,Fan, Fenliang,Yin, Chang,Li, Zhaojun,Song, Alin,Liang, Yongchao,Wan, Yunfan,Liang, Yongchao.

[16]Bacterial Diversity in the Digestive Tracts of Four Indian Air-Breathing Fish Species Investigated by PCR Based Denaturing Gradient Gel Electrophoresis. He, Suxu,Zhou, Zhigang,Huang, Lu,Banerjee, Goutam,Ray, Arun Kumar,Ringo, Einar. 2016

[17]Genetically modified WYMV-resistant wheat exerts little influence on rhizosphere microbial communities. Zhang, Zhenhua,Liu, Yan,Yu, Cigang,Dong, Shanshan,Wang, Changyong,Bao, Wangbo,Yu, Ran,Chen, Ming.

[18]Histological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strains. Salma, Wahida,Askarian, Fatemeh,Kousha, Armin,Ringo, Einar,Zhou, Zhigang,Wang, Wenwen,Kousha, Armin,Ebrahimi, Maryam Tajabadi,Myklebust, Reidar. 2011

[19]The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere. Zhang, Baoguo,Bai, Zhihui,Tang, Ling,Zhuang, Guoqiang,Hoefel, Daniel,Wang, Xiaoyi,Li, Baoju,Li, Zuming.

[20]Gut microbial status induced by antibiotic growth promoter alters the prebiotic effects of dietary DVAQUA (R) on Aeromonas hydrophila-infected tilapia: Production, intestinal bacterial community and non-specific immunity. Zhou, Zhigang,He, Suxu,Liu, Yuchun,Cao, Yanan,Meng, Kun,Yao, Bin,Ringo, Einar,Yoon, Ilkyu. 2011

作者其他论文 更多>>