数字农科院2.0

半矮生型桃F-box基因的克隆和表达特征分析

文献类型： 中文期刊

作者： 王玉兰;鲁振华;王志强;牛良;崔国朝

作者机构：

关键词： 半矮生型桃;‘SD9238’;F-box;基因克隆;RACE;qRT-PcR

期刊名称：果树学报

ISSN： 1009-9980

年卷期： 2013 年 30 卷 04 期

页码： 543-550

收录情况： 北大核心 ; CSCD

摘要： 【目的】研究半矮生型桃‘SD9238’F-box基因的序列特征及F-box基因在新梢生长跃变期的表达与节间伸长的关系,为进一步研究该基因调控半矮生型桃植株生长的分子机制奠定基础。【方法】利用基因克隆和RACE技术获得‘SD9238’F-box基因的gDNA及cDNA序列,并分析该基因的序列特征。采用qRT-PCR分析该基因在新梢生长跃变期的表达水平。【结果】获得F-box基因gDNA全长3 650 bp,cDNA全长1 936 bp,GeneBank登陆号为KF023192。该基因ORF长1 509 bp编码502个氨基酸,蛋白质分子质量55.984 kD。生物信息学分析表明,氨基酸序列具有F-box蛋白家族中LRR的结构特征,与蔷薇科中苹果和草莓的F-box基因同源,相似性达98%。QRT-PCR分析结果表明,F-box基因在节间生长跃变期表达量的动态变化与新梢节间的伸长速率动态变化趋势一致,其基因相对表达量在半矮生型桃中于节间生长跃变点5月22日达到最大值,为第一时期的9.4倍;在普通型桃中同样于5月22日达到基因相对表达量的最大值,但只为第一时期的2.2倍。【结论】获得了‘SD9238’新梢中F-box基因KF023192的序列全长,并明确了该基因的序列特征。对该基因在新梢生长过程的动态表达分析显示,KF023192可能参与了半矮生桃新梢节间生长的调控。

分类号： S662.1

  • 相关文献

[1]柔嫩艾美耳球虫真核起始因子5和5A基因的克隆表达及转录动态分析. 苟灵俏,龚振兴,于三科,殷昊,马雪婷,蔡建平,林青. 2014

[2]核桃FRUITFULL(JrFUL)基因的克隆及表达分析. 王企,陈利娜,夏小丛,敬丹,李好先,骆翔,曹尚银. 2019

[3]棉花GhDMT7基因对5-氮杂胞嘧啶核苷的响应机制. 杨志宁,陆许可,范亚朋,孙玉平,喻欣,王亮,高永健,古丽加依那什·哈不力,古丽沙西·拿斯依,吾木尔·阿不都,黄慧,张梦豪,王立东,陈筱,肖磊,张鑫蕊,王帅,陈修贵,王俊娟,郭丽雪,高文伟,叶武威. 2024

[4]甜菜BvCK2基因全长cDNA的克隆和序列分析. 鲁振强,潘彦遥,陈连江,朱延明,李春宇. 2009

[5]基于表达序列标签(EST)策略的茶树分子生物学研究. 陈亮,赵丽萍,马春雷,张亚丽. 2007

[6]棉铃虫蓝光受体基因cryptochromes的克隆鉴定. 徐蓬军,吴孔明. 2011

[7]猪ADAR1基因cDNA全长克隆、序列信息及组织表达分析. 张跃博,欧阳峰正,王立刚,侯欣华,刘欣,颜华,张龙超,王立贤. 2019

[8]紫花苜蓿LEA第3组基因(MsLEA3-1)的克隆与分析. 金后聪,杨青川,孙彦,康俊梅,晁跃辉,金洪,龙瑞才,李燕. 2010

[9]棉花一个新F-box基因的克隆与表达分析. 卫江辉,范术丽,宋美珍,庞朝友,喻树迅. 2011

[10]F-box only protein (FBXO) family genes in cattle (Bos taurus): Genome-wide identification and functions in the skeletal muscle development. Fengcheng Song,Chen Gao,Yufei Zan,Yu Sun,Tingting Wang,Zhepei Zhang,Lupei Zhang,Zhengrong Yuan,Tianliu Zhang,Xue Gao. 2025

[11]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[12]Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake. Pu, L.,Zhang, L. C.,Zhang, J. S.,Wang, L. G.,Liang, J.,Zhang, Y. B.,Liu, X.,Yan, H.,Zhang, T.,Yue, J. W.,Wang, L. X.,Song, X.,Li, N.,Wu, Q. Q.. 2016

[13]Cloning and characterization of gene encoding a Mn-containing superoxide dismutase in Eutrema halophilum. Xu, X. J.,Zhou, Y. J.,Bu, H. H.,Feng, J. C.,Ren, D. T.,Wang, G. Y.. 2014

[14]Cloning and Characterization of a Putative CTR1 Gene from Wheat. Bi Cai-Li,Wen Xiao-Jie,Zhang Xue-Yong,Liu Xu,Bi Cai-Li. 2010

[15]Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum. Chen Shuai,Liu Guan-shan,Wang Yuan-ying,Sun Yu-he,Chen Jia. 2011

[16]Molecular cloning and characterization of hatching enzyme-like gene in the silkworm, Bombyx mori. Tang, Shun-ming,Shen, Xing-jia,Zhao, Qiao-ling,Zhang, Guo-zheng,Guo, Xi-jie,Lu, Fu-hao,Wang, Na.

[17]Identification, bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa. Xiao, Bang,Zhang, Xingju,Li, Yong,Tang, Zhonglin,Yang, Shulin,Mu, Yulian,Cui, Wentao,Ao, Hong,Li, Kui,Xiao, Bang. 2009

[18]Gene cloning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thuringiensis insecticidal Cry1Ac toxin in Helicoverpa armigera (Lepidoptera : Noctuidae). Wang, GR,Liang, GM,Wu, KM,Guo, YY. 2005

[19]Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters. Li, Jiaqi,Wang, Liangliang,Zhang, Hao,Chen, Songling,Mei, Yingjie,Wang, Chong,Ling, Fei,Du, HongLi,Fang, Wei,Chen, Yaosheng,Liu, Xiaohui. 2010

[20]Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hubner). Wu, KM,Guo, YYN. 2004

作者其他论文 更多>>