数字农科院2.0

网箱养殖青石斑鱼Epinephelusawoara鳃及体表粘附菌群的PCR-DGGE比较分析

文献类型: 中文期刊

作者: 刘玉春;周志刚;石鹏君;何夙旭;姚斌;韩少锋

作者机构:

关键词: 青石斑鱼;DGGE;粘附菌群;指纹图谱

期刊名称:中国农业科技导报

ISSN:

年卷期: 2008 年 01 期

页码: 81-86

收录情况: CSCD

摘要: 采用免培养的16SrDNA梯度凝胶电泳技术(denaturinggradientgelelectrophoresis,DGGE)对海水网箱养殖青石斑鱼Epinephelusawoara鳃和体表粘附菌群结构进行了比较分析。结果表明青石斑鱼鳃粘附菌群结构相对简单,存在绝对优势种群,体表粘附菌群结构较为复杂,无绝对优势种群,聚类分析表明青石斑鱼鳃与体表粘附菌群结构存在较大差异性(相似度52%),而个体间鳃或体表的粘附菌群结构相似性较好。测序结果表明青石斑鱼鳃与体表粘附菌群以未培养菌为主,鳃的绝对优势菌为Pantoeasp.,体表相对优势菌为Meio-thermussp.、Unculture...

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