数字农科院2.0

辣椒RAPD扩增体系的建立

文献类型： 中文期刊

作者： 谢立波;郭亚华;孟凡娟;刘录祥;王雪;高永利

作者机构：

关键词： 辣椒;RAPD;优化

期刊名称：安徽农业科学

ISSN： 0517-6611

年卷期： 2010 年 38 卷 34 期

页码： 19272-19274

收录情况： 北大核心

摘要： [目的]为辣椒的品种资源鉴定与遗传多样性分析奠定基础。[方法]采用改良的CTAB法对辣椒叶片基因组DNA进行提取,并采用正交试验设计法对辣椒RAPD-PCR反应进行优化筛选。[结果]采用改良的CTAB法获得了高质量的辣椒基因组DNA,用Tris-平衡酚∶氯仿∶异戊醇(25∶24∶1,V∶V∶V)进行抽提,最后再用氯仿抽提以除去其中含有的酚,避免了对后续PCR扩增的影响。基于一般的RAPD反应程序和调整试验,优化了PCR反应体系。结果表明,在模板20 ng 1.5μl、引物(20μmol/L)1.5μl、dNTPs(2.5 mmol/L)2.2μl、10×Buffer缓冲液(含Mg2+)2.5μl、Taq(5 U/μl)0.35μl条件下扩增效果较好。[结论]建立了最适的辣椒RAPD扩增体系。

分类号： S641.3

  • 相关文献

[1]辣椒经地面模拟诱变后的RAPD鉴定. 郭亚华,谢立波,王雪,陈立新,刘录祥,周宇. 2014

[2]辣椒抗疫病研究中的RAPD反应体系优化. 胡勇胜,毛胜利,刘明月,张宝玺. 2007

[3]野火球RAPD反应体系优化研究初报. 安晓珂,赵来喜,郭树春,李继平. 2008

[4]紫花苜蓿RAPD反应条件优化. 孙杰,杨青川,韩国栋. 2010

[5]RAPD分子标记鉴定紫花苜蓿品种的反应体系优化. 张涛,杨青川,毛培胜. 2006

[6]紫花苜蓿RAPD反应条件优化. 孙杰,杨青川,韩国栋. 2002

[7]小麦RAPD分析中温度循环参数的优化. 牛永春,钟鸣. 2001

[8]GeneticdiversityanalysisofMagnaporthegriseafromsomeblastnurseriesofHunanprovinceusingrandomamplifiedpolymorphicDNA. NguekoRB,沈瑛,王洪凯,林福呈,徐同. 2004

[9]Random amplified polymorphism of DNA analysis in Aegilops tauschii. Kong, LR,Dong, YC,Jia, JZ. 1998

[10]Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis. Dwivedi, SL,Gurtu, S,Chandra, S,Yuejin, W,Nigam, SN. 2001

[11]A new strategy employed for identification of sweet orange cultivars with RAPD markers. Sun, X.,Mu, Q.,Wang, C.,Wang, X. C.,Fang, J. G.,Jiang, D.. 2012

[12]RAPD study on inter-species relationships in Roegneria (Poaceae : Triticeae). Zhou, YH,Yang, JL,Zheng, YL,Yan, J,Jia, JZ,Wei, YM. 1999

[13]RAPD analysis of jasmine rice-specific genomic structure. Wu, Y. J.,Chen, Y.,Wang, J.,Zhu, C. X.,Xu, B. L.. 2006

[14]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[15]Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid between N-tabacum and N-glauca. Sun, Y.,Xue, Q.,Zhang, X.,Ding, C.,Zhang, L.,Gong, M.,Bu, G.,Wang, W.. 2007

[16]IDENTIFICATION OF AN RAPD MARKER TIGHTLY LINKED TO BLAST RESISTANCE IN ORYZA-SATIVA L. LU, J,QIAN, HR,ZHUANG, JY,LIN, HX,ZHENG, KL. 1995

[17]Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Zhang, Ruiying,Huang, Chenyang,Zheng, Suyue,Zhang, Jinxia,Ng, Tzi Bun,Jiang, Ruibo,Zuo, Xuemei,Wang, Hexiang.

[18]Quantitative trait loci underlying the development of seed composition in soybean (Glycine max L. Merr.). Li, Wenbin,Sun, Desheng,Du, Yuping,Chen, Qingshan,Zhang, Zhongchen,Qiu, Lijuan,Sun, Genlou.

[19]EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.. Zhang, Caiyun,Shi, Wenshuo,Ma, Keshi,Zhang, Feixiong,Li, Hongjie. 2016

[20]A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers. Zhang Guang-qing,Qi Jian-min,Zhang Xiao-chen,Fnag Ping-ping,Tao Ai-fen,Lan Tao,Wu Wei-ren,Zhang Guang-qing,Qi Jian-min,Wu Wei-ren,Su Jian-guang,Liu Ai-min,Liu Ai-min. 2011

作者其他论文 更多>>