亲和层析纯化棘球蚴囊液特异性抗原的研究
文献类型: 中文期刊
作者: 贾万忠;刘卫;刘金凤;张克益;田广孚
作者机构:
关键词: 亲和层析;兔抗健康羊IgG吸附柱;兔抗羊细颈囊尾蚴IgG吸附柱;绵羊棘球;蚴包囊液(SHCF);ELISA
期刊名称:中国兽医科技
ISSN: 1000-6419
年卷期: 1991 年 05 期
页码:
摘要: 本文应用兔抗健康羊IgG吸附柱、兔抗羊细颈囊尾蚴IgG吸附柱分别对绵羊棘球蚴囊液粗抗原进行了亲和吸附,制得了亲和纯抗原,并用ELISA法测定其抗原性。试验表明,经过兔抗羊细颈囊尾蚴IgG吸附柱吸附后的抗原,与非疫区绵羊血清反应的OD值都低,而与疫区血清反应的OD值都较高。经过兔抗健康羊IgG吸附柱吸附后,在不影响阳性检出率的情况下,可以降低阴性血清所致的假阳性反应,同时提高了细颈囊尾蚴的检出率。
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