数字农科院2.0

牛毛囊基因组DNA制备方法的比较研究

文献类型: 中文期刊

作者: 刘玲;付强;朱化彬;彭秀丽;郝海生;杜卫华;赵学明;王栋

作者机构:

关键词: DNA;裂解法;皮埃蒙特牛;南阳牛;扩增检测;南牛;水煮法;于非;引物序列;伤害性

期刊名称:中国畜牧兽医学会动物繁殖学分会第十五届学术研讨会论文集(上册)

ISSN:

年卷期: 2010 年

页码: 5

收录情况: CSCD

摘要: 由于非伤害性采样的重要性和明显优势,毛囊样品已经成为一种非常重要的非伤害采样形式,为解决其基因组DNA难于制备和PCR扩增易受抑制等问题,本试验比较了水煮法、DTT裂解法、蛋白酶K裂解法制备哺乳动物毛囊中基因组DNA后PCR扩增的检测效果。结果表明,蛋白酶K裂解法在制备毛囊基因组DNA时显示出了快速、高效、经济、安全的优点。并根据扩增检测结果推测,蛋白酶K裂解法不但有利于基因组DNA的释放,而且可以消除毛囊中可能存在的PCR扩增色素抑制剂,为以后科学地进行毛囊样品的采集及毛囊基因组DNA的制备提供了理论依据。

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