数字农科院2.0

苹果锈果类病毒的几种提取方法及RT-PCR检测灵敏度的比较

文献类型: 中文期刊

作者: 郭瑞;李世访;白容霖;成卓敏

作者机构:

关键词: 苹果锈果类病毒;RT-PCR;NA-PEX;快速提取RNA

期刊名称:植物保护

ISSN: 0529-1542

年卷期: 2006 年 32 卷 02 期

页码: 95-97

收录情况: 北大核心

摘要: 以感染苹果锈果类病毒的苹果枝条为材料,利用不同的方法抽提,进行RT-PCR检测。比较了6种RNA的提取方法,结果发现:LiCl法、RNeasy plant mini kit和NA-PEX法检测效果较佳。RNeasy plant mini kit可检测到10-2,NA-PEX可检测到10-1;RNeasy plant mini kit检测效果好于NA-PEX,但价格比较贵,需要研磨;NA-PEX法组织无需液氮研磨,试剂便宜,耗时短,适合检测大批量的样品。

分类号: S432.41

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