数字农科院2.0

豌豆种传花叶病毒抗病基因sbm-1的RAPD标记

文献类型: 中文期刊

作者: 李汝刚;范云六;N.F.Weeden

作者机构:

关键词: 豌豆;豌豆种传花叶病毒;RAPD

期刊名称:科学通报

ISSN: 0023-074X

年卷期: 1996 年 18 期

页码: 1712-1714

收录情况: 北大核心 ; CSCD

摘要: 与抗病性状连锁的DNA标记对抗病后代的选择和抗病基因的克隆提供了非常有用的工具。豌豆种传花叶病毒(PSbMV)是由种子,并经蚜虫非持久性传播的病毒,有3个株系:P-1,L及P-4,其中以P-1株系分布最广。系列研究表明:4个隐性基因控制着对3个株系的抗性,其中sbm-1对P-1,sbm-2及sbm-3对L及其相关的L-1,而sbm-4则对P-4。1993年新西兰的Timmerman首次报道了sbm-1的RFLP(restriction fragment length polymorphism)标记,但遗传距离较大,为8cM。本研究根据BSA(bullked segregant analysis)方法,鉴定了与sbm-1连锁的RAPD(random amplified polymorphic DNA)标记。

分类号: S436.43

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