转基因抗虫棉对根区土壤真菌影响的初步研究
文献类型: 中文期刊
作者: 谢响明;李世东;缪作清;张永军
作者机构:
关键词: 转基因抗虫棉;土壤;真菌;DGGE;风险评价
期刊名称:微生物学杂志
ISSN:
年卷期: 2008 年 04 期
页码: 7-12
收录情况: CSCD
摘要: 以转基因抗虫棉SGK321、GK12及亲本对照石远321、泗棉三号为材料,连续2a利用平板涂布法测定棉花根区土壤真菌数量及主要类群数量的变化,并在第2a蕾期和花期对真菌进行DGGE检测。结果显示:第1a全部供试品种真菌数量均随生育期增加,第2a真菌数量变化趋势与上年不同,2a相同生育期内转基因棉和亲本间均存在差异:①SGK321与石远321相比,第1a蕾期、吐絮期真菌数量及吐絮期镰刀菌数量差异显著;②GK12与泗棉三号相比,第1a花期真菌数量及蕾期、花期的毛霉数量差异显著;第2a蕾期真菌数量及青霉数量差异显著。DGGE结果显示,在蕾期和花期,转基因棉与亲本相比,真菌数量及主要类...
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