适合鸭瘟病毒弱毒增殖的MHC单倍型鸭胚肝间充质干细胞的建立
文献类型: 中文期刊
作者: 佟相慧;;问婉欣;;陈洪岩;;韩凌霞
作者机构:
关键词: 鸭瘟病毒;鸭胚肝间充质干细胞;主要组织相容性复合体单倍型
期刊名称:中国兽医科学
ISSN: 1673-4596
年卷期: 2020 年 NULL 卷 2 期
页码: 183-188
摘要: 为了建立适于鸭瘟病毒(DPV)弱毒增殖的细胞系,利用4个主要组织相容性复合体(MHC)单倍型鸭品系,体外分离鸭胚肝细胞,在干细胞因子、白细胞抑制因子和成纤维细胞生长因子诱导下,获得鸭胚肝间充质干细胞(DuLMSC).接种DPV疫苗株C-KCE后,结果显示:F1~F10代均能稳定出现CPE;将F10病毒接种细胞24 h后,通过扫描电镜在胞质、胞核及细胞间隙均能够观察到典型的疱疹病毒粒子;F1至F10代均能扩增出DPV UL2基因片段,序列测定结果表明F1、F5、F10代的扩增产物与NCBI中公布的DPV株(EU082088)的核苷酸同源性为100%;组织培养半数感染量和一步生长曲线结果表明,源自B4系单倍型鸭的DuLMSC细胞系增殖DPV的病毒含量明显高于B1、B2和B3系.本研究为稳定培养鸭瘟病毒提供了优良稳定的实验材料.
分类号: S858.32
- 相关文献
[1]鸭瘟病毒在鸭胚脐带间充质干细胞上生物学特性的研究. 徐丽晶,陆涛峰,王怡平,陈洪岩,赵丽丽. 2017
[2]Ⅰ型鸭肝炎病毒和鸭瘟病毒一步法二重RT-PCR检测方法的建立. 徐丽晶,赵丽丽,刘海燕,王怡平,陈洪岩. 2018
[3]鸭瘟病毒疫苗株基因组复制非必需区的筛选. 胡玉珍,陈普成,丁蕾蕾,赵玉博,姜永萍,陈化兰,柳金雄. 2018
[4]鸭瘟重组病毒穿梭载体的构建及分析. 刘柏含,牛银杰,赵丽丽,弥春霞,陈洪岩. 2017
[5]鸭瘟病毒作为非复制型疫苗载体的研究. 李慧昕,王玉龙,韩宗玺,刘胜旺. 2014
作者其他论文 更多>>
-
Regional projections and sampling strategies for the global germplasm resources collection of Oryza rufipogon Griff.
作者:王恺;;龙金花;;雷济舟;;梁云涛;;钱前;;郑晓明
关键词:Oryza rufipogon Griff.; Germplasm resource collection; Genetic diversity; Environmental factor; MaxEnt model
-
High-density cultivation of synthetic apomictic hybrid rice achieves near-complete 1 diploidization and parental-equivalent yield in field trials 2 Mengqiu Song1,5, Shengnan Li1,5, Zuan Wang1,5, Guojun Dong2 , Tao Wang1 , Jingxi Sun1 ,
作者:宋梦秋;;李胜男;;王钻;;董国军;;王涛;;孙景熙;;夏思琪;;杨泽园;;刘伟哲;;彭友林;;樊龙江;;孙健;;张勇;;王克剑;;郑晓明;;钱前
关键词:cultivation;;hybrid rice;;near-complete ;;diploidization;;parental-equivalent
-
Genome-wide identification of the Medicago ruthenica FTIP gene family and expression profiling under salt stresses
作者:田永雷;;朱琳;; 郭茂伟;;李志勇;; 武自念;;李鸿言;; 李星月;;王晓龙
关键词:FT-interacting proteins; Medicago ruthenica; genome-wide identification; salt stress; saline–alkali stress
-
Deciphering the ceRNA Network in Alfalfa: Insights into Cold Stress Tolerance Mechanisms
作者:朱琳;;赵宇杰;;郭茂伟;;白杰;;张良斌;;李志勇
关键词:alfalfa; cold stress; mRNAS; ncRNAs; ceRNAs
-
The threshold of aeration intensity in micro-oxygen lactic acid fermentation and the underlying microbial mechanisms
作者:曹起涛;;尹福斌;;连天境;;周谈龙;;蔡阳扬;;魏晓曼;;孙佳欣;;董红敏
关键词:agricultural waste; lactic acid; micro-oxygen; aeration threshold; microbial function
-
Strengthening global rice germplasm sharing: insights from the International Network for the Genetic Evaluation of Rice (INGER) platform
作者:樊佳雨;;夏思琪;;杨泽园;;郑晓明;;陈帆
关键词:ice germplasm resources · INGER · Sharing mechanism · Sustainable agricultural development
-
Contrasting effects of three aging processes on arsenic immobilization in red versus black soils amended by cerium-manganese modified biochar: the unique role of freeze-thaw cycling in governing arsenic fate at micro/nano interfaces
作者:吕鹏;;黄晓雅;;李莲芳*;;焦艳
关键词:Modifed biochar; Aging mechanism; Arsenic fate; Freeze–thaw cycling; Soil micro-interfaces
