数字农科院2.0

应用RFLP和DGGE技术对沼气池中产甲烷菌多样性的研究

文献类型: 中文期刊

作者: 徐彦胜;阮志勇;刘小飞;胡国全

作者机构:

关键词: 产甲烷菌;群落多态性;mcrA克隆文库;RFLP;DGGE

期刊名称:西南农业学报

ISSN: 1001-4829

年卷期: 2010 年 23 卷 04 期

页码: 1319-1324

收录情况: 北大核心 ; CSCD

摘要: 利用PCR-RFLP和PCR-DGGE技术对处于正常运行阶段的4口农村户用沼气池进行研究,考查其中产甲烷菌的种群结构并对其进行系统发育分析。特异性地扩增产甲烷菌mcrA片段和16S rDNA序列分别进行RFLP分析和DGGE分析。对RFLP得到的每一个独特的OTU(operational taxonomic units)和DGGE中的优势条带进行测序及序列分析。结果表明,不同发酵原料的沼气池中产甲烷菌的优势类群存在差异性,但又有一定的相似性。同时研究结果显示,2种不同实验方法得出的结论有着某些一致性。

分类号: S216.4

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