数字农科院2.0

尤力克柠檬上一种新病害的生物学特性及RT-PCR检测

文献类型： 中文期刊

作者： 陈洪明;王雪峰;周彦;周常勇;郭俊;李中安

作者机构：

关键词： 柠檬;柑橘黄脉病;生物学特性;嫁接传播;RT-PCR

期刊名称：植物保护学报

ISSN： 0577-7518

年卷期： 2015 年 42 卷 04 期

页码： 557-563

收录情况： 北大核心 ; CSCD

摘要： 近年我国云南瑞丽市的一果园尤里克柠檬上出现一种引起叶片皱缩、反卷或呈船形,叶背水浸状、侧脉脉明、黄化的柑橘新病害。为明确该病害的病原及其生物学特性,通过田间观察、病样接种、电镜观察,并基于柑橘黄脉病毒(Citrus yellow vein clearing virus,CYVCV)外壳蛋白基因设计引物对病样进行RT-PCR检测。结果表明,该病是一种嫁接传染性病害,能侵染柠檬和酸橙品种且症状明显,并能摩擦接种到辣椒和豇豆等草本植物。该病发病最适温度为18~24℃。春梢嫩叶症状表现明显,夏梢和秋梢基本不表现症状,而晚秋梢也表现出较春梢稍弱的典型症状。引致该病的病毒为(13~15)nm×(400~1 000)nm大小的联合丝状病毒。RT-PCR扩增产物为614 bp,且其与CYVCV外壳蛋白序列相似性达97%以上,表明该病害是由CYVCV引起的柑橘黄脉病,并初步建立了该病的RT-PCR检测技术。

分类号： S436.669

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