应用AFLP-DNA指纹技术鉴定梅花品种的研究
文献类型: 中文期刊
作者: 明军;张启翔;晏晓兰;邱丽娟
作者机构:
关键词: 梅花;AFLP;DNA 指纹;品种鉴定
期刊名称:北京林业大学学报
ISSN: 1000-1522
年卷期: 2003 年 S2 期
页码: 17-22
收录情况: 北大核心 ; CSCD
摘要: 应用银染法AFLP技术,通过筛选的7对Mse I-EcoR I引物组合,建立了162个梅花品种的175个样品指纹。使用NTSYSpc2.1t软件,计算遗传相似性,以SAHN Clustering进行不加权成对算术平均法(UPGMA)聚类分析。其中引物组合E-ACT/M-CAG和E-GA/M-CTC分别仅有两个样品不能区分开,其余引物组合均能鉴别175个样品中的164个以上的样品。7对引物组合的平均鉴别效率达到95.89%,两对引物组合可以鉴别所有样品。以引物组合E-GA/M-CTC为例进行了品种鉴定的聚类分析。所以,用AFLP-DNA指纹图谱来鉴别梅花品种资源,效率是很高的。
分类号: S685.17
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