数字农科院2.0

猪瘟Erns基因在大肠杆菌中的高效表达

文献类型: 中文期刊

作者: 贾洪林;仇华吉;李娜;朱庆虎;罗玉子;童光志

作者机构:

关键词: 猪瘟病毒;RT-PCR;原核表达

期刊名称:中国预防兽医学报

ISSN: 1008-0589

年卷期: 2005 年 27 卷 3 期

页码: 161-163

收录情况: CSCD

摘要: 提取猪瘟病毒石门系强毒株的基因组RNA,用RT-PCR扩增其Erns基因的cDNA,将其克隆到原核表达载体pPROEXTMHTc中,然后转化大肠杆菌DH5α,经IPTG诱导,Erns基因获得高效表达.SDS-PAGE分析显示,表达的重组融合蛋白表现分子量约为31 ku.Western blot分析表明,重组蛋白具有良好的免疫原性.重组蛋白可用于制备诊断抗原,用于标记疫苗接种和野毒感染的鉴别诊断.

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