数字农科院2.0

采用 CRISPR/Cas9 创制黄籽高抗裂角油菜杂交种

文献类型： 中文期刊

关键词： 甘蓝型油菜; BnaTT8; BnaIND; CRISPR/Cas9

期刊名称：分子植物育种

ISSN： 1672-416X

年卷期： 2024 年

页码：

收录情况： 北大核心（2023版） ; ; 科技核心（2024版） ; ; 农林核心（2020版）

摘要： Resistance to pod shattering is crucial for the mechanized harvesting of rapeseed, making both yellow seed and shatter resistance important breeding targets. In order to improve the yellow seed and crack resistance traits of 'Dadi199' by using gene editing technology. This study identified the genetic basis of the yellow seed trait in the rapeseed hybrid 'Dadi199' by examining the expression of key genes in the seed coat color metabolic pathway in its parents, 'Z11' (Zhongshuang11) and the yellow-seeded 'R11'. Using CRISPR/Cas9 gene-editing technology, we created mutant lines of 'R11' with combined mutations in BnaIND (INDEHISCENT) and BnaTT8 (TRANSPARENT TESTA 8), and crossed these with previously created mutant lines of 'Z11' with mutations in the same genes. The hybrid combinations were evaluated for pod shattering resistance and quality traits using 'Dadi199' as a control. The hybrid combinations of the mutants exhibited strong resistance to pod shattering (shattering resistance index of 1.00) and prominent yellow seed traits, with an average oil content of 50.38%, which is a 2.45% increase over the commercial seed oil content of 'Dadi199'. This study used gene editing technology to rapidly develop a hybrid variety with high resistance to pod shattering and yellow seeds, providing a theoretical basis for the genetic improvement of rapeseed quality and traits suitable for mechanization.

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