数字农科院2.0

粳稻花时性状 QTL 定位与聚合效应分析

文献类型: 中文期刊

关键词: 粳稻;;花时;;重组自交系;;BSA-seq;;QTL 定位

期刊名称:中国水稻科学

ISSN: 1001-7216

年卷期: 2025 年

页码:

收录情况: 北大核心(2023版) ; ; CSCD(2025-2026年度) ; ; 科技核心(2024版) ; ; 农林核心(2024版)

摘要: 【目的】水稻日开花时间是影响粳稻不育系异交率及制繁种产量的重要性状。本研究旨在定位粳稻花时调控相关的QTL并对其聚合效应加以分析,明确早花时 QTL的主效位点。【方法】利用早花时粳稻保持系粳 65B(J65B)和晚花时粳稻保持系粳 139B(J139B)构建包含 221 个家系的重组自交系群体。连续 3 年调查群体花时性状,采用遗传图谱和 BSA-seq两种方法进行花时 QTL 定位分析。【结果】 利用完备区间作图法共检测到 30 个花时性状QTL,分布于 2-12 号染色体上,其中 qFT5.2、qFT5.3、qFT5.5、qFT10.2、qFT10.3 在 8 个以上环境中被同时检测到,加性效应范围 13.04-50.93,是稳定表达的主效位点;选取花时极端早和极端晚家系构建混池,利用 BSA-seq 方法在 qFT5.3、FT5.5、qFT6.2、qFT10.2 位点检测到花时关联区域;选取两种方法共同定位到的稳定表达位点进行 QTL 聚合分析,J65B类型的 QTLs 均会提早花时,qFT5.3 和 qFT6.2 是早花时改良的基础位点,qFT5.5、qFT10.2是进一步提早花时的重要位点。【结论】采用两种方法定位到 4 个稳定表达主效花时 QTLqFT5.3、qFT5.5、qFT6.2、qFT10.2,为早花时基因进一步精细定位、基因克隆和粳稻花时性状改良奠定了基础。

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