数字农科院2.0

基于 13 C 脉冲标记揭示施氮对牧草光合碳分配的影响

文献类型： 中文期刊

关键词： 13C 脉冲标记;;施氮;;苜蓿;;燕麦;;光合碳分配

期刊名称：中国农业科学

ISSN： 0578-1752

年卷期： 2025 年

页码：

收录情况： 北大核心（2023版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： 【目的】研究施氮对苜蓿和燕麦的生物量、13C 丰度和 13C 固定量的影响，揭示植物-土壤系统碳分配规律，探究提升土壤碳固存和改善土壤质量的有效方法，为北方农牧交错带土地利用与生态环境治理提供理论依据。【方法】试验采用 13C 脉冲标记法，以苜蓿和燕麦为研究对象，设置施加氮肥（75 kg·hm-2）和对照（不施氮）处理。通过对苜蓿和燕麦地上、地下生物量以及不同器官 13C 丰度分析，研究施氮对作物光合碳分配的影响。【结果】与不施氮相比，施氮提升燕麦土壤有机碳含量，对苜蓿土壤有机碳含量无明显影响；施氮处理显著提高苜蓿茎叶和根系生物量，分别增加 117.5%和 97.8%。燕麦对氮肥响应较小，地上和地下生物量分别增加 19.1%和 9.6%；施氮处理显著提高苜蓿植株整体 13C 丰度，提高效果优于燕麦，两者分别提高 54.36‰和 28.6‰；施氮处理提升了 13C 在苜蓿根系中的分配比例，从 5.0%提升至 11.5%，而在燕麦-土壤系统中，施氮对 13C 在茎叶、根系和根际土壤中分配比例影响不显著，根系 13C 的分配比例由 46.1%增至 47.1%。【结论】施氮均可提升苜蓿与燕麦土壤有机碳含量，对苜蓿生物量有促进作用，对燕麦生物量影响不显著；施氮能有效提高苜蓿植株 13C 固定量，并提升 13C 在苜蓿根系中分配比例，促进光合碳向地下部分转移，但施氮对燕麦 13C 固定量及 13C 在燕麦各组分中分配比例影响不显著。

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