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一株H5N6亚型高致病性禽流感病毒遗传演化及生物学特性研究

文献类型： 中文期刊

关键词： H5N6亚型;;高致病性禽流感病毒;;分子特征;;遗传演化;;抗原性;;哺乳动物致病性

期刊名称：中国预防兽医学报

ISSN： 1008-0589

年卷期： 2025 年

页码：

收录情况： 北大核心（2023版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： In May 2023,one H5N6 highly pathogenic avian influenza virus(HPAIV),named as A/chicken/Jiangsu/S1/2023 (H5N6),abbreviated as CK/JS/S1/2023(H5N6),was isolated from poultry.To further understand the biological characteristics of this virus,we conducted whole genome sequencing,phylogenetic analysis,and reassortment analysis using Simplot software. Antigenicity was assessed via hemagglutination inhibition assays.The results indicated that the HA protein of the virus contains a cleavage site at PLRERRRKR↓GLF,consistent with the molecular characteristics of HPAIVs.Analysis of key amino acid sites revealed that mutations S133A and T156A in the receptor-binding domain of the HA protein enhance the virus's ability to bind to human type receptors(α2,6).Additionally,a deletion of amino acids aa59 to aa69 was identified in the stalk region of the NA protein. Mutations associated with increased pathogenicity in mammals were also observed,including D622G in the PB1 protein,I43M and T215A in the M1 protein,and I106M in the NS1 protein.Phylogenetic analysis revealed that the HA gene belongs to subclade 2.3.4.4b,while the NA gene and five internal genes(PB2,PB1,PA,NP,M)are of the Eurasian lineage,and the NS gene belongs to the allele A branch.Reassortment analysis showed that this virus is a complex reassortant strain,originating from H5N8 in wild birds and H5N6, H4N6,and H3N2 in domestic ducks.Antigenicity analysis indicated that the virus antigenically matches the antisera induced by the H5-Re14 seed virus of the subclade 2.3.4.4b vaccine.To assess the virus's pathogenecity,mice were intranasally infected with doses ranging from 101EID50/50μL to 106EID50/50μL,and the survival rates and viral titers of each organ of the mice were measured. Infected mice displayed clinical symptoms such as lethargy,ruffled fur,and weight loss,with mortality beginning on the fourth day post-infection.Pathogenicity experiments in mice demonstrated that the virus could efficiently replicate in multiple organs without prior adaptation,with the highest viral titers observed in the lungs,reaching 7.25log10EID50/mL.The median lethal dose(MLD50)for mice was 1.5log10EID50,indicating high pathogenicity(MLD50<3log10EID50).This study provides valuable data on the biological characteristics of the H5N6 HPAIV,which will contribute to the ongoing efforts for the prevention and control of subclade 2.3.4.4b H5 subtype HPAI.

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