数字农科院2.0

Antibody Responses and the Vaccine Efficacy of Recombinant Glycosyltransferase and Nicastrin Against Schistosoma japonicum

文献类型： 外文期刊

作者： Bowen Dong;Haoran Zhong;Danlin Zhu;Luobin Wu;Jinming Wang;Hao Li;Yamei Jin

作者机构：

关键词： immune protection;Schistosoma japonicum;SjGT;SjNCSTN

期刊名称： Pathogens

ISSN： 2076-0817

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Schistosomiasis is a neglected tropical disease and the second most common parasitic disease after malaria. While praziquantel remains the primary treatment, concerns about drug resistance highlight the urgent need for new drugs and effective vaccines to achieve sustainable control. Previous proteomic studies from our group revealed that the expression of Schistosoma japonicum glycosyltransferase and nicastrin as proteins was higher in single-sex males than mated males, suggesting their critical roles in parasite reproduction and their potential as vaccine candidates. In this study, bioinformatic tools were employed to analyze the structural and functional properties of these proteins, including their signal peptide regions, transmembrane domains, tertiary structures, and protein interaction networks. Recombinant forms of glycosyltransferase and nicastrin were expressed and purified, followed by immunization experiments in BALB/c mice. Immunized mice exhibited significantly elevated specific IgG antibody levels after three immunizations compared to adjuvant and PBS controls. Furthermore, immunization with recombinant glycosyltransferase and nicastrin significantly reduced the reproductive capacity of female worms and liver egg burden, though egg hatchability and adult worm survival were unaffected. These findings demonstrate that recombinant glycosyltransferase and nicastrin are immunogenic and reduce female worm fecundity, supporting their potential as vaccine candidates against schistosomiasis.

分类号：

  • 相关文献

[1]Schistosoma japonicum translationally controlled tumour protein, which is associated with the development of female worms, as a target for control of schistosomiasis. Haoran Zhong,Yuqi Ren,Fanglin Qin,Xiaochun Li,Ling Hou,Shaopeng Gu,Yamei Jin. 2022

[2]Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Cheng Guofeng,Lin Jiaojiao,Sun Jun,Li Hao,Zhu Chuangang,Zhou Yuancong,Cai Youmin. 2005

[3]Preparation and immunoprotection of subgroup B avian leukosis virus inactivated vaccine. Li, Xue,Dong, Xuan,Sun, Xiaolong,Li, Weihua,Zhao, Peng,Cui, Zhizhong,Li, Weihua,Wang, Xin. 2013

[4]Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. Peng, Wei,Si, Wei,Yin, Lu,Liu, Huifang,Yu, Shenye,Liu, Siguo,Wang, Chunlai,Chang, Yuehong,Zhang, Zhuo,Hu, Shouping,Du, Yanfen. 2011

[5]Recombinant adenovirus expressing type Asia1 foot-and-mouth disease virus capsid proteins induces protective immunity against homologous virus challenge in mice. Zhou, Guohui,Wang, Haiwei,Wang, Fang,Yu, Li.

[6]Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. Tan, Lei,Liao, Ying,Fan, Jin,Zhang, Yuqiang,Mao, Xiang,Sun, Yingjie,Song, Cuiping,Qiu, Xusheng,Meng, Chunchun,Ding, Chan,Ding, Chan.

[7]Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model. Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua,Wang, Xiao,Yao, Junhu,Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua. 2015

[8]Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine. Zhongwang Zhang,Fei Yao,Jianliang Lv,Yaozhong Ding,Xinsheng Liu,Liping Zhang,Zhongyuan Ma,Peng Zhou,Yonglu Wang,Huichen Guo,Li Pan. 2023

[9]Recombinant Toxoplasma gondii ribosomal protein p2 modulates the functions of murine macrophages in vitro and provides immunity against acute toxoplasmosis in vivo. Yu Zhengqing,Lu Yujia,Liu Zhaoyi,Aleem Muhammad Tahir,Liu Junlong,Luo Jianxun,Yan Ruofeng,Xu Lixin,Song Xiaokai,Li Xiangrui. 2021

[10]Nano DNA vaccine encoding toxoplasma gondii histone deacetylase SIR2 enhanced protective immunity in mice. Zhengqing Yu,Yujia Lu,Wandi Cao,Muhammad Tahir Aleem,Junlong Liu,Jianxun Luo,Ruofeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li. 2021

[11]Toxoplasma gondii proteasome subunit alpha type 1 with chitosan: A promising alternative to traditional adjuvant. Zhengqing Yu,Wenxi Ding,Muhammad Tahir Aleem,Junzhi Su,Junlong Liu,Jianxun Luo,Ruofeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li. 2021

[12]Identification and characterization of a novel protective antigen, SeseC_00396 of Streptococcus equi ssp. Zooepidemicus. Li Yi,Weiping Yang,Jinpeng Li,Shaohui Wang,Yang Wang. 2021

[13]With Chitosan and PLGA as the Delivery Vehicle, Toxoplasma gondii Oxidoreductase-Based DNA Vaccines Decrease Parasite Burdens in Mice. Zhengqing Yu,Wandi Cao,Xuchen Gao,Muhammad Tahir Aleem,Junlong Liu,Jianxun Luo,Ruofeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li. 2021

[14]PipC affects the virulence of Salmonella enterica serovar Enteritidis and its deletion strain provides effective immune protection in mice. Zhang, Lu,Chen, Yubin,Yan, Zhigang,Li, Yuntai,Yang, Xiaowen,Chen, Li,Zhang, Yanying,Chen, Yingyu,Li, Yonghui,Shi, Qiumei,Wu, Tonglei. 2025

[15]Review of advanced research on swine Actinobacillus pleuropneumoniae vaccine development strategy. Adehanom Baraki Tesfaye,Rui Han,Zhengyu Tao,Liuchao You,Jiayao Zhu,Pengcheng Gao,Lei Fu,Yuefeng Chu. 2025

[16]Studies on the expression of the 28kD glutathione S-transferase gene from Schistosoma japonicum in the silkworm (Bombyx mori) larvae and insect cells. Tian, E,Yang, GZ,Cai, YM,Shi, FH,Wu, XF. 1997

[17]Proteome-wide analysis of lysine acetylation in adult Schistosoma japonicum worm. Hong, Yang,Cao, Xiaodan,Han, Qian,Yuan, Chunxiu,Zhu, Chuangang,Lu, Ke,Li, Hao,Fu, Zhiqiang,Lin, Jiaojiao,Zhang, Min,Han, Yanhui,Lin, Jiaojiao,Lin, Tao.

[18]Prevention of Schistosoma japonicum infection in mice with long-acting praziquantel implants. Lei, Lei,Guo, Shengrong,Cheng, Liang,Hou, Jingwen,Zhu, Chuangang,Shi, Yaojun,Jiang, Yan,Lin, Jiaojiao. 2012

[19]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[20]Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing. Cheng, GF,Lin, JJ,Feng, XG,Fu, ZQ,Jin, YM,Yuan, CX,Zhou, YC,Cai, YM. 2005

作者其他论文 更多>>