数字农科院2.0

Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus

文献类型： 外文期刊

作者： Haibing Liang;Bingqian Zhou;Zhe Hu;Xiaoyu Chu;Xuefeng Wang;Cheng Du;Xiaojun Wang

作者机构：

关键词： AC-ELISA;EIAV;p26

期刊名称： Microorganisms

ISSN： 2076-2607

年卷期： 2025 年 13 卷 7 期

页码：

收录情况： SCIE（2025版）

摘要： Equine Infectious Anemia Virus (EIAV) poses significant diagnostic challenges due to its genetic variability and the limitations of conventional nucleic acid detection methods. This study developed an antigen-capture, enzyme-linked immunosorbent assay (AC-ELISA) for the detection and quantification of the EIAV capsid protein p26. The assay utilized a monoclonal antibody (1G11) specific to the p26 protein as the capture antibody and a polyclonal antibody as the detection antibody, forming a highly specific and sensitive detection system. Under optimized conditions, the detection limit of the AC-ELISA was 1.95 ng/mL, with a good linear relationship observed between 1.95 ng/mL and 60.5 ng/mL of p26 protein. Additionally, the AC-ELISA effectively distinguished EIAV from other equine viruses, including equine herpesvirus 1 (EHV-1), equine arteritis virus (EAV), and equine influenza virus (EIV), without cross-reactivity. Importantly, the AC-ELISA demonstrated the ability to detect multiple EIAV strains, including virulent strains, attenuated strains, and strains from other countries, highlighting its broad applicability across diverse EIAV isolates. Compared to western blot and reverse transcriptase assays, the AC-ELISA exhibited higher sensitivity and strong correlation in quantifying the EIAV p26 protein. The assay is simple, rapid, and cost-effective, making it suitable for both laboratory research and clinical applications. It provides a powerful tool for EIAV detection and quantification, supporting future vaccine development and clinical trials.

分类号：

  • 相关文献

[1]Development of antigen capture ELISA for the quantification of EIAV p26 protein. Hu, Zhe,Chang, Hao,Ge, Man,Lin, Yuezhi,Wang, Xuefeng,Guo, Wei,Wang, Xiaojun. 2014

[2]Identification and characterization of a common B-cell epitope on EIAV capsid proteins. Hu, Zhe,Chang, Hao,Chu, Xiaoyu,Li, Shuang,Wang, Meiyue,Wang, Xiaojun.

[3]Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia. Hu, Zhe,Guo, Kui,Du, Cheng,Sun, Jinhui,Naletoski, Ivancho,Chu, Xiaoyu,Lin, Yuezhi,Wang, Xuefeng,Barrandeguy, Maria,Samuel, Mino,Wang, Wen,Lau, Patrick Imtung,Wernery, Ulrich,Raghavan, Rekha,Wang, Xiaojun. 2023

[4]传染性法氏囊病超强毒株Gx的分离鉴定及分子生物学和抗原性分析. 王笑梅,付朝阳,高宏雷,宋秀龙,曾祥伟. 2002

[5]马传染性贫血弱毒疫苗诱导的细胞免疫应答特点与免疫保护相关性研究. 林跃智,邓喜林,沈楠,吕晓玲,赵立平,邵一鸣,沈荣显,周建华. 2008

[6]以中国马传染性贫血病毒减毒疫苗为模型探索慢病毒免疫保护机理. 马建,林跃智,王雪峰,何翔,贾斌,梁华,沈弢,孟庆来,杜承,周建华,沈荣显,邵一鸣,王晓钧. 2014

[7]马传染性贫血病毒Gag p9蛋白功能研究进展. 魏丽丽,王晓钧,王盈,李景鹏,相文华,沈荣显. 2004

[8]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[9]马传染性贫血病毒(EIAV)嵌合感染性分子克隆的体外构建. 涂亚斌,王柳,刘红全,仇华吉,童光志. 2001

[10]EIAV强毒株和弱毒疫苗株gp90基因差异与免疫原性关系研究. 王雪峰,杨彬,王帅,林跃智,吕晓玲,赵立平,王凤龙,周建华. 2008

[11]EIAV疫苗弱毒株诱导的超感染抵制现象与其诱导天然免疫激活间的相关性研究. 马建,王珊珊,林跃智,王雪峰,杜承,周建华. 2012

[12]EIAV驴白细胞弱毒疫苗株减毒过程第61代毒前病毒全基因组DNA的克隆与分析. 赵薇薇,王雪峰,马建,吕晓玲,赵玉军,周建华. 2008

[13]EIAV减毒疫苗克隆株诱导体液免疫反应的时相研究. 阳凯,孟庆来,陈新江,马燕,相文华,张耀洲,邵一鸣,张晓燕. 2009

[14]马传贫病毒主要基因变异研究进展. 耿庆华,相文华. 2005

[15]带有分子标记的马传染性贫血病毒弱毒株的构建. 温建新,童光志,仇华吉,涂亚斌,吴东来,王柳. 2003

[16]免疫抑制对EIAV弱毒疫苗株在免疫马体内载量的影响. 马建,姜成刚,林跃智,郭亮,沈荣显,邵一鸣,周建华. 2008

[17]马疱疹病毒1/4型双重PCR分型检测方法的建立. 郭巍,田志革,王晓钧,相文华. 2012

[18]马传染性贫血病毒Gagp9蛋白功能研究进展. 魏丽丽,王晓钧,王盈,李景鹏,相文华,沈荣显. 2004

[19]马传染性贫血发病过程中细胞毒性T细胞的作用及其表位研究. 马建,孔宪刚,沈荣显,周建华. 2006

[20]EIAV Rev蛋白拮抗eqTRIM5α介导的AP-1信号通路的机制研究. 宋丹丹,那雷,王晓钧. 2020

作者其他论文 更多>>