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Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

文献类型： 外文期刊

作者： Ji, Yujie;Gao, Bo;Zhao, Dan;Wang, Yao;Zhang, Lu;Wu, Han;Xie, Yifan;Shi, Qiuyu;Guo, Wei

作者机构：

关键词： bioinformatics analysis;expression pattern;ROS level;bacterial load;insecticidal activity;Spodoptera exigua

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2024 年 72 卷 4 期

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： The p38 mitogen-activated protein kinases (MAPKs) are associated with insect immunity, tissue repair, and the insecticidal activity of Bacillus thuringiensis (Bt). Here, a p38 MAPK family gene (Sep38 beta) was identified from Spodoptera exigua. Among the developmental stages, the transcription level of Sep38 beta was the highest in egg, followed by that in prepupa and pupa. Sep38 beta expression peaked in Malpighian tubules and the hemolymph of fifth instar larvae. Knockdown of Sep38 beta or injection of SB203580 (a p38 MAPK inhibitor) significantly downregulated the SeDUOX expression and reactive oxygen species (ROS) level in the midgut, accounting for deterioration of the midgut to scavenge pathogens and enhancement of Bt insecticidal activity. In conclusion, all the results demonstrate that Sep38 beta regulates the immune-related ROS level in the insect midgut, which suppresses the insecticidal activity of Bt against S. exigua by 17-22%. Our study highlights that Sep38 beta is essential for insect immunity and the insecticidal activity of Bt to S. exigua and is a potential target for pest control.

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