数字农科院2.0

Profiles of antibiotic- and heavy metal-related resistance genes in animal manure revealed using a metagenomic analysis

文献类型： 外文期刊

作者： Chong Liu;Guichun Li;Xiaobo Qin;Yansheng Xu;Jiarui Wang;Guosheng Wu;Huabing Feng;Jing Ye;Changxiong Zhu;Xiaohua Li;Xiangqun Zheng

作者机构：

关键词： Antibiotic-resistance gene;Co-selection;Heavy metal;Metagenomic sequencing;Metal-resistance gene

期刊名称： Ecotoxicology and Environmental Safety

ISSN： 0147-6513

年卷期： 2022 年 239.0 卷

页码：

收录情况： SCIE（2022版）

摘要： Farmed animals produce excrement containing excessive amounts of toxic heavy metals as a result of consuming compound feed as well as receiving medical treatments, and the presence of these heavy metals may aggravate the risk of spreading drug-resistance genes through co-selection during manure treatment and application processes. However, research on the association between heavy metals and antimicrobial resistance is still lacking. In this study, metagenomic sequencing was used to explore the effects of the co-selection of environmentally toxic heavy metals on the resistome in manure. A relevance network analysis showed that metal-resistance genes (MRGs), especially for copper (Cu) and zinc (Zn), were positively correlated with multiple types of antibiotic-resistance genes (ARGs) and formed a complex network. Most bacteria that co-occurred with both MRGs and ARGs simultaneously are members of Proteobacteria and accounted for 54.7% of the total microbial species in the relevance network. The remaining bacteria belonged to Firmicutes, Bacteroidetes and Actinobacteria. Among the four phyla, Cu- and Zn-resistance genes had more complex correlations with ARGs than other MRG types, reflecting the occurrence of ARG co-selection under the selective pressure of high Cu and Zn levels. In addition, approximately 64.8%, 59.1% and 68.4% of MRGs that correlated with the presence of plasmids, viruses and prophages, respectively, are Cu- or Zn-resistant, and they co-occurred with various ARGs, indicating that mobile genetic elements participate in mediating ARG co-selection in response to Cu and Zn pressure. The results indicated that the use of heavy-metal additives in feed induces the increases of drug resistance genes in manure through co-selection, aggravating the risk of antimicrobial resistance diffusion from animal farm to manure land applications.

分类号：

  • 相关文献

[1]Complete sequence of the multi-resistance plasmid pV7037 from a porcine methicillin-resistant Staphylococcus aureus. Wendlandt, Sarah,Schwarz, Stefan,Li, Beibei,Ma, Zhiyong. 2013

[2]Characterization of heavy metal, antibiotic pollution, and their resistance genes in paddy with secondary municipal-treated wastewater irrigation. Qing Yan,Zhengzheng Zhong,Xiaoyan Li,Zhaoyun Cao,Xiaolong Zheng,Guozhong Feng. 2024

[3]A review of the resistome within the digestive tract of livestock. Tao Ma,Tim A. McAllister,Le Luo Guan. 2021

[4]Metagenomic insights into the composition and function of the gut microbiota of mice infected with Toxoplasma gondii. Jin Xin Meng,Xin Yu Wei,Huanping Guo,Yu Chen,Wei Wang,Hong Li Geng,Xing Yang,Jiang Jiang,Xiao Xuan Zhang. 2023

[5]Differential Microbial Communities in Paddy Soils Between Guiyang Plateaus and Chengdu Basins Drive the Incidence of Rice Bacterial Diseases. Wang, Yajiao,Tian, Shuping,Wu, Nan,Liu, Wenwen,Li, Li,Wang, Xifeng. 2022

[6]Characteristics of psychrophilic bacterial communities and associated metabolism pathways in different environments by a metagenomic analysis. Wen Jun Xu,Lu Meng,Yan Kun Zhao,Jie Wu,Hui Min Liu,Jia Qi Wang,Nan Zheng. 2024

[7]Soybean productivity can be enhanced by understanding rhizosphere microbiota: evidence from metagenomics analysis from diverse agroecosystems. Ren, Honglei,Hong, Huilong,Zha, Bire,Lamlom, Sobhi F.,Qiu, Hongmei,Cao, Yongqiang,Sun, Rujian,Wang, Haorang,Ma, Junkui,Zhang, Hengbin,Sun, Liping,Yang, Qing,Zhou, Changjun,Liu, Xiulin,Wang, Xueyang,Zhang, Chunlei,Zhang, Fengyi,Zhao, Kezhen,Yuan, Rongqiang,Abdelghany, Ahmed M.,Zhang, Bixian,Zheng, Yuhong,Wang, Jiajun,Lu, Wencheng. 2025

[8]Metagenomics reveals differences in spore-forming bacterial diversity in raw milk in different regions and seasons in China. Ning Li,Wenjun Xu,Lu Meng,Yankun Zhao,Xiaowei Zhao,Nan Zheng,Yangdong Zhang,Jiaqi Wang. 2024

[9]Exploring the Impact of Dinotefuran Residue on Microbial Community and Flavor Generation in Huangjiu Fermentation. Zhao, Shanshan,Xu, Qisi,Li, Minmin,Chen, Jieyin,Francis, Frederic,Dai, Xiaofeng,Tan, Jianxin,Kong, Zhiqiang. 2025

[10]Seasonal dynamics and risk assessment of antibiotic resistome in straw-retained agroecosystems. Yi Wu,Yi Fan,Yiheng Tao,Quankuan Guo,Shuang Pang,Wei Yang,Ximei Zhang. 2025

[11]Cd, Cu, And Zn Accumulations C.aused By Long-Term Fertilization I n Greenhouse Soils And Their Potential Risk Assessment. Chen, Yali,Islam, Md. Shafiqul,Weng, Liping,Li, Yongtao,Ma, Jie,Liao, Zhongbin,Li, Yongtao. 2019

[12]Effects of Mn-Cd antagonistic interaction on Cd accumulation and major agronomic traits in rice genotypes by different Mn forms. Huang, Qi-na,Yang, Yong-jie,Liang, Yan,Shao, Guo-sheng,An, Hua.

[13]Research of Heavy Metals Determination in Cereals by Inductively Coupled Plasma Mass Spectrometry. Wang Bu-jun. 2012

[14]Effect of aeration rate, moisture content and composting period on availability of copper and lead during pig manure composting. Shen, Yujun,Zhao, Lixin,Meng, Haibo,Hou, Yueqing,Zhou, Haibin,Cheng, Hongsheng,Shen, Yujun,Zhao, Lixin,Meng, Haibo,Hou, Yueqing,Zhou, Haibin,Cheng, Hongsheng,Wang, Fei,Liu, Hongbin. 2016

[15]Accumulation, availability, and uptake of heavy metals in a red soil after 22-year fertilization and cropping. Zhou, Shiwei,Xu, Minggang,Sun, Nan,Liu, Jing,Lv, Jialong.

[16]Cd accumulation and subcellular distribution in two ecotypes of Kyllinga brevifolia Rottb as affected by Cd treatments. Hao, Xiaoqing,Li, Tingxuan,Yu, Haiying,Zhang, Xizhou,Zheng, Zicheng,Chen, Guangdeng,Zhang, Shujin,Zhao, Li,Pu, Yong,Hao, Xiaoqing,Zhang, Shujin,Pu, Yong.

[17]The influence of pH and organic matter content in paddy soil on heavy metal availability and their uptake by rice plants. Ouyang, Younan,Zhang, Guoping,Zeng, Fanrong,Ali, Shafaqat,Zhang, Haitao,Qiu, Boyin,Wu, Feibo.

[18]Association Study Reveals Genetic Loci R.esponsible For Arsenic, Cadmium A nd Lead Accumulation In Rice Grain In Contaminated Farmlands. Chen, Sunlu,Qin, Ping,Zheng, Guangyong,Xu, Xiangyang,Shi, Xiaoliang,Teng, Sheng,Peng, Yu,Liu, Xiuyan,Liu, Xiuyan,Liu, Xiuyan,Chen, Sunlu,Chen, Mingxue. 2019

[19]Source attributions of heavy metals in rice plant along highway in Eastern China. Feng, Jinfei,Zhao, Jian,Zhu, Liqun,Bian, Xinmin,Zhang, Weijian,Wang, Yinxi,Zhang, Weijian.

[20]Selected dark sides of biomass-derived biochars as environmental amendments. Chen, Zien,Luo, Lei,Xiao, Diyi,Lv, Jitao,Wen, Bei,Zhang, Shuzhen,Ma, Yibing.

作者其他论文 更多>>