数字农科院2.0

Phenotypic and comparative transcriptomic analyses of resistant and susceptible germplasm reveal the putative resistance mechanisms of wheat to fusarium crown rot

文献类型： 外文期刊

作者： Meng Zhang;Dongmei Li;Lifeng Gao;Mingyue He;Guoguo Lv;Eryong Chen;Lei Zhang;Xiaojia Su;Haoyang Ding;Xueli Wu;Chunji Liu;Haiyan Hu

作者机构：

关键词： EMS-induced germplasm X413;Fusarium crown rot;Resistance;Transcriptome;Wheat

期刊名称： BMC Genomics

ISSN： 1471-2164

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Fusarium crown rot (FCR), primarily caused by Fusarium pseudograminearum, has emerged as a globally significant disease severely threatening the stability of wheat production. Breeding FCR-resistant germplasms and clarifying the underlying resistance mechanisms are critical prerequisites for effective disease management. Results: In this study, to generate genotypes with enhanced FCR resistance, an EMS-mutagenized population was developed from AK58, and a germplasm X413 with stably moderate resistance to FCR at both the seedling and adult stages was identified. Phenotypic analysis showed that X413 exhibited stronger ability to inhibit the expansion and mycelial growth of F. pseudograminearum. Transcriptome (RNA-seq) comparison with highly susceptible X73 revealed that X413 had fewer differentially expressed genes (DEGs) after pathogen infection, with its specific DEGs significantly enriched in resistance-related pathways such as “lignin metabolic process” and “phenylpropanoid biosynthesis”. In contrast, X73 had more DEGs, and genes related to growth and development including those for DNA replication and post-replication repair were significantly downregulated, consistent with its more obvious plant height reduction after pathogen infection. Weighted gene co-expression network analysis (WGCNA) identified hub genes highly expressed in X413, including ubiquitin-related genes, kinase genes, and three germin-like protein genes (with SOD activity involved in hydrogen peroxide production). Physiological assays confirmed that X413 had significantly higher hydrogen peroxide content and SOD activity than X73. Conclusions: Collectively, FCR resistance in X413 may be associated with efficient activation of disease resistance pathways and balanced growth-defense metabolism. This germplasm could serve as a valuable resistance source to support wheat breeding for FCR resistance, and the mechanistic insights obtained may also lay a solid theoretical foundation for the mining and utilization of resistance genes.

分类号：

  • 相关文献

[1]Transcriptome Dynamics in Triticum aestivum Genotypes Associated with Resistance against the Wheat Dwarf Virus. Abdoallah Sharaf,Przemysław Nuc,Jan Ripl,Glenda Alquicer,Emad Ibrahim,Xifeng Wang,Midatharahally N. Maruthi,Jiban Kumar Kundu. 2023

[2]An Improved Inoculation Method to Detect Wheat and Barley Genotypes for Resistance to Fusarium Crown Rot. Jinlong Li,Xiangru Xu,Yanling Ma,Qixin Sun,Chaojie Xie,Jun Ma. 2022

[3]充分发掘保护利用生物多样性;促进农业可持续发展——《生物多样性与小麦改良》简评. 李祥洲. 1997

[4]甘蓝型油菜BnEIN3基因的克隆及菌核病诱导表达研究(英文). 许李明,黄军艳,刘学群,覃瑞,刘胜毅. 2009

[5]Homo logs to Cry toxin receptor genes in a de novo transcriptome and their altered expression in resistant Spodoptera litura larvae. Gong, Liang,Wang, Huidong,Qi, Jiangwei,Hu, Meiying,Han, Lanzhi,Jurat-Fuentes, Juan Luis.

[6]Down-Regulation Of Aminopeptidase N And A.bc Transporter Subfamily G T ranscripts In Cry1Ab And Cry1Ac Resistant Asian Corn Borer, Ostrinia Furnacalis (Lepidoptera: Crambidae). Zhang, TT,Coates, BS,Wang, YQ,Wang, YD,Bai, SX,Wang, ZY,He, KL. 2017

[7]Combined metabolomic transcriptomic analysis reveals the role of flavonoid pathway in white rot resistance of Vitis davidii Foex. grapes. Xibei Tan,Peng Li,Hao Wang,Liyuan Gu,Ruitao Liu,Jianfu Jiang,Lei Sun,Xiucai Fan,Chonghuai Liu,Ying Zhang. 2024

[8]Integrative Analysis of Transcriptomics and Proteomics for Screening Genes and Regulatory Networks Associated with Lambda-Cyhalothrin Resistance in the Plant Bug Lygus pratensis Linnaeus (Hemiptera: Miridae). Jing Chen,Zhi Jia Huo,Fei Long Sun,Li Qi Zhang,Hai Bin Han,Jiang Zhu,Yao Tan. 2025

[9]The wheat AGC kinase TaAGC1 is a positive contributor to host resistance to the necrotrophic pathogen Rhizoctonia cerealis. Zhu, Xiuliang,Yang, Kun,Wei, Xuening,Rong, Wei,Du, Lipu,Ye, Xingguo,Qi, Lin,Zhang, Zengyan,Zhang, Qiaofeng.

[10]Microsatellite marker identification of a Triticum aestivum Aegilops umbellulata substitution line with powdery mildew resistance. Zhu, Zhendong,Zhou, Ronghua,Kong, Xiuying,Kong, Xiuying,Dong, Yuchen,Jia, Jizeng. 2006

[11]Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat. Li, Zhao,Xu, Huijun,Du, Lipu,Zhang, Zengyan,Zhou, Miaoping.

[12]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[13]The wheat calcium-dependent protein kinase TaCPK7-D positively regulates host resistance to sharp eyespot disease. Wei, Xuening,Shen, Fangdi,Hong, Yantao,Rong, Wei,Du, Lipu,Liu, Xin,Xu, Huijun,Ma, Lingjian,Zhang, Zengyan,Shen, Fangdi,Ma, Lingjian.

[14]Genetic evidence of local adaptation of wheat yellow rust (Puccinia striiformis f. sp tritici) within France. Duan, X,Leconte, M,Hovmoller, MS,De Vallavieille-Pope, C.

[15]Optimization of breeding methods when introducing multiple resistance genes from American to Chinese wheat. Qi, Jinfeng,Lin, Feng,Zhang, Xuecai,Yin, Changbin,Li, Huihui. 2011

[16]Mapping of a wheat resistance gene to yellow mosaic disease by amplified fragment length polymorphism and simple sequence repeat markers. Nie, H,He, ZT,Chen, XL,Han, YP,Wang, JR,Li, X,Han, CG,Yu, JL. 2005

[17]Genetic dissection of the seed dormancy trait in cultivated rice (Oryza sativa L.). Wan, JM,Jiang, L,Tang, JY,Wang, CM,Hou, MY,Jing, W,Zhang, L.

[18]Response of wheat germplasm to infestation of english grain aphid (Hemiptera: Aphididae). Li, Fengqi,Chen, Liang,Peng, Junhua,Li, Fengqi,Kong, Lingrang,Liu, Yusheng,Wang, Hezhou,Peng, Junhua.

[19]30个小麦新品系抗条锈病基因分析及成株期抗病性评价. 冯晶,蔺瑞明,林凤,徐世昌. 2013

[20]小麦抗菌蛋白编码基因BS108的克隆及其转基因抗病小麦的创制. 李钊,周淼平,张增艳,庄洪涛,徐惠君,杜丽璞,叶兴国. 2010

作者其他论文 更多>>