数字农科院2.0

Two B-Box Proteins, GhBBX21 and GhBBX24, Antagonistically Modulate Anthocyanin Biosynthesis in R1 Cotton

文献类型： 外文期刊

作者： Shuyan Li;Kunpeng Zhang;Chenxi Fu;Chaofeng Wu;Dongyun Zuo;Hailiang Cheng;Limin Lv;Haiyan Zhao;Jianshe Wang;Cuicui Wu;Xiaoyu Guo;Guoli Song

作者机构：

关键词： anthocyanin biosynthesis;BBX proteins;cotton

期刊名称： Plants

ISSN： 2223-7747

年卷期： 2025 年 14 卷 15 期

页码：

收录情况： SCIE（2025版）

摘要： The red plant phenotype of R1 cotton is a genetic marker produced by light-induced anthocyanin accumulation. GhPAP1D controls this trait. There are two 228 bp tandem repeats upstream of GhPAP1D in R1 cotton. In this study, GUS staining assays in transgenic Arabidopsis thaliana (L.) Heynh. demonstrated that tandem repeats in the GhPAP1D promoter-enhanced transcriptional activity. GhPAP1D is a homolog of A. thaliana AtPAP1. AtPAP1’s expression is regulated by photomorphogenesis-related transcription factors such as AtHY5 and AtBBXs. We identified the homologs of A. thaliana AtHY5, AtBBX21, and AtBBX24 in R1 cotton, designated as GhHY5, GhBBX21, and GhBBX24, respectively. Y1H assays confirmed that GhHY5, GhBBX21, and GhBBX24 each bound to the GhPAP1D promoter. Dual-luciferase reporter assays revealed that GhHY5 weakly activated the promoter activity of GhPAP1D. Heterologous expression assays in A. thaliana indicated that GhBBX21 promoted anthocyanin accumulation, whereas GhBBX24 had the opposite effect. Dual-luciferase assays showed GhBBX21 activated GhPAP1D transcription, while GhBBX24 repressed it. Further study indicated that GhHY5 did not enhance GhBBX21-mediated transcriptional activation of GhPAP1D but alleviates GhBBX24-induced repression. Together, our results demonstrate that GhBBX21 and GhBBX24 antagonistically regulate anthocyanin accumulation in R1 cotton under GhHY5 mediation, providing insights into light-responsive anthocyanin biosynthesis in cotton.

分类号：

  • 相关文献

[1]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[2]2015年全国棉花种植品种监测报告——播种品种(系)344个;数量减少83个;减幅19.4%. 毛树春,冯璐,芦建华. 2016

[3]2015年中国与美国棉花种植品种比较. 冯璐,毛树春. 2016

[4]兰州百合病毒病原的DAS-ELISA检测. 王发林,古勤生,刘芬,彭斌,刘丽锋. 2003

[5]Synthesis Of Seed-Specific Bidirectional Promoters F.or Metabolic Engineering Of A nthocyanin-Rich Maize. Li, Suzhen,Chen, Rumei,Yang, Wenzhu,Liu, Xiaoqing,Mu, Bona,Jiao, Yong,Fan, Yunliu,Jiao, Yong,Zhou, Xiaojin,Zhang, Chunyi. 2018

[6]Engineering Of 'Purple Embryo Maize' W.ith A Multigene Expression S ystem Derived From A Bidirectional Promoter And Self-Cleaving 2A Peptides. Zhang, Chunyi,Chen, Rumei,Zhou, Xiaojin,Fan, Yunliu,Yang, Wenzhu,Li, Suzhen,Li, Ye,Mu, Bona,Liu, Xiaoqing. 2018

[7]The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation. An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,Tian, Yi. 2012

[8]Identification of anthocyanin biosynthesis related microRNAs in a distinctive Chinese radish (Raphanus sativus L.) by high-throughput sequencing. Sun, Yuyan,Qiu, Yang,Duan, Mengmeng,Wang, Jinglei,Zhang, Xiaohui,Wang, Haiping,Song, Jiangping,Li, Xixiang,Sun, Yuyan.

[9]Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes. Xinkang Feng,Aminu Shehu Abubakar,Kunmei Chen,Chunming Yu,Aiguo Zhu,Jikang Chen,Gang Gao,Xiaofei Wang,Pan Mou,Ping Chen. 2023

[10]The Light-Induced WD40-Repeat Transcription Factor DcTTG1 Regulates Anthocyanin Biosynthesis in Dendrobium candidum. Ning Jia,Jingjing Wang,Yajuan Wang,Wei Ye,Jiameng Liu,Jinlan Jiang,Jing Sun,Peipei Yan,Peiyu Wang,Fengzhong Wang,Bei Fan. 2021

[11]DcTT8, a bHLH transcription factor, regulates anthocyanin biosynthesis in Dendrobium candidum. Ning Jia,Jing-Jing Wang,Jiameng Liu,Jinlan Jiang,Jing Sun,Peipei Yan,Yufeng Sun,Peiyu Wan,Wei Ye,Bei Fan. 2021

[12]Genome wide characterization of R2R3 MYB transcription factor from Apocynum venetum revealed potential stress tolerance and flavonoid biosynthesis genes. Aminu Shehu Abubakar,Xinkang Feng,Gang Gao,Chunming Yu,Jikang Chen,Kunmei Chen,Xiaofei Wang,Pan Mou,Deyi Shao,Ping Chen,Aiguo Zhu. 2022

[13]Synthesis Of Seed-Specific Bidirectional Promoters For Metabolic Engineering Of A nthocyanin-Rich Maize. Liu, XQ, Li, SZ, Yang, WZ, Mu, BN, Jiao, Y, Zhou, XJ, Zhang, CY, Fan, YL, Chen, RM. 2018

[14]Light-Induced TaHY5-7A and TaBBX-3B Physically Interact to Promote PURPLE PERICARP-MYB 1 Expression in Purple-Grained Wheat. Qinqin Jiang,Wenhui Jiang,Ning Hu,Rui Tang,Yuxuan Dong,Hongqi Wu,Tianxiang Liu,Lulu Guan,Hanbing Zhang,Junbin Hou,Guaiqiang Chai,Zhonghua Wang. 2023

[15]Genome-Wide Identification of the MYB and bHLH Families in Carnations and Expression Analysis at Different Floral Development Stages. Luhong Leng,Xiaoni Zhang,Weichao Liu,Zhiqiang Wu. 2023

[16]Systematic analysis of MYB transcription factors and the role of LuMYB216 in regulating anthocyanin biosynthesis in the flowers of flax (Linum usitatissimum L.). Dong wei XIE,Jing LI,Xiao yu ZHANG,Zhi gang DAI,Wen zhi ZHOU,Jian guang SU,Jian SUN. 2023

[17]Regulation of anthocyanin synthesis in red lettuce in plant factory conditions: A review. Hadiqa Anum,Kun Li,Javaria Tabusam,Said Abdelhalim Abdelaty Saleh,Rui feng Cheng,Yu xin Tong. 2024

[18]Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis. Caiming Gou,Jia Li,Bo Chen,Guoting Cheng,Zhangfei Zheng,Hua Peng,Ahmed H. El-Sappah. 2025

[19]AaMYB61-like and AabHLH137 jointly regulate anthocyanin biosynthesis in Actinidia arguta. Zhe Song,Yukuo Li,Xu Zhan,Xiaohan Li,Lingshuai Ye,Miaomiao Lin,Ran Wang,Leiming Sun,Jinyong Chen,Jinbao Fang,Feng Wei,Xiujuan Qi. 2025

[20]Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta). Yukuo Li,Zhe Song,Xu Zhan,Xiaohan Li,Lingshuai Ye,Miaomiao Lin,Ran Wang,Hailei Huang,Jian Guo,Leiming Sun,Hong Gu,Jinyong Chen,Jinbao Fang,Xiujuan Qi. 2025

作者其他论文 更多>>