数字农科院2.0

A novel recombination porcine epidemic diarrhea virus isolated from Gansu, China: Genetic characterization and pathogenicity

文献类型： 外文期刊

作者： Li L.;Li B.;Wang J.;Liu L.;Li Y.;Sun S.;Yin S.;Zhang L.;Liu X.;Xu X.;Guo H.

作者机构：

关键词： Molecular epidemiology;Pathogenicity;PEDV;S protein;Vaccine

期刊名称： Veterinary Microbiology

ISSN： 0378-1135

年卷期： 2024 年 290 卷

页码：

收录情况： SCIE（2024版）

摘要： Porcine epidemic diarrhea virus (PEDV) is an acute and highly contagious porcine enteric coronavirus. It has caused serious economic losses of pig industry in China. Here we insolated a current PEDV field strain named GS2022, analyzed the characters of genetic variation and pathogenicity. The results demonstrated that the GS2022 strain was belong to a newly defined subgroup G2 d, forming an independent branch which mainly contains strains isolated in China from 2017 to 2023. Notably, there are multiple mutations and extensive N-glycosylation compared to CV777 strain and PT-P5 strain, therefore the structure of GS2022 strain is different from 6U7K and 7W6M. Animal pathogenicity test showed that GS2022 strain could cause severe clinical signs and the high level of virus shedding in 7-day-old piglets. But recovery of diarrhea after 5 days, and no pathological damage to important organs. Further study on 3-day-old piglets also indicated GS2022 strain have pathogenicity. In this study no piglets died, which make it possible for that GS2022 strain become a candidate vaccine. These results are helpful to understand the epidemiology, molecular characteristics, evolution, and antigenicity of PEDV circulating in China. It also provides reference for designing effective vaccines against PEDV. © 2024 Elsevier B.V.

分类号：

  • 相关文献

[1]An oral recombinant human type 5 adenovirus vector vaccine encoding the S protein of Type I feline coronavirus effectively protection against FCoV challenge in cats. Gu nan Deng,Chang zhan Xie,Ke lei Zhou,Chun sai He,Yuan Zhen Ma,A. jia Ake,Rui Guo,Kai Li,Chen Peng,Bin Zhang. 2025

[2]Next-Generation Vaccines for Co-Circulating PEDV and TGEV: Integrating Nucleic Acid Platforms, Mucosal Delivery, and AI–Driven Antigen Design. Xiaojun Hu,Zhenshan Wang,Shen Wang,Hongyu Sun,Na Feng,Entao Li,Xianzhu Xia,Guixue Hu,Feihu Yan. 2025

[3]Emerging natural recombinant Marek's disease virus between vaccine and virulence strains and their pathogenicity. Zhang, Yanping,Lan, Xingge,Wang, Yanan,Lin, Yumeng,Yu, Zhenghao,Guo, Rongrong,Li, Kai,Cui, Hongyu,Qi, Xiaole,Wang, Yongqiang,Gao, Li,Pan, Qing,Liu, Aijing,Gao, Yulong,Wang, Xiaomei,Liu, Changjun. 2022

[4]Pathogenicity Evaluation and Virulence Gene Identification of an Attenuated Duck Enteritis Virus. Xiaona Shi,Haibin Zhuang,Dun Shuo,Luzhao Li,Shenghui Pan,Zihua Wu,Mei Tang,Wenxia Yang,Qinfang Liu,Chunxiu Yuan,Dawei Yan,Xue Pan,Bangfeng Xu,Zhifei Zhang,Minghao Yan,Qiaoyang Teng,Zejun Li. 2025

[5]A Virulent Systemic Feline Calicivirus Strain Isolated in China Could Produce Broadly Neutralizing Antibodies Against Multiple Strains. Wuchang Heng, Dan Zang, Ruiyu Li, Ruibin Qi, Qian Jiang, Jiasen Liu, Honglin Jia, and Hongtao Kang. 2025

[6]马流感灭活疫苗的研制. 郭巍,戚亭,相文华,王晓钧. 2015

[7]Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Cheng Guofeng,Lin Jiaojiao,Sun Jun,Li Hao,Zhu Chuangang,Zhou Yuancong,Cai Youmin. 2005

[8]ProtectiveimmunityinducedbyaDNAvaccineexpressingeIF4AofToxoplasmagondiiagainstacutetoxoplasmosisinmice. 陈佳,SiyangHuang,ZhongyuanLi,ZiguoYuan,DonghuiZhou,EskildPetersen,NianzhangZhang,XingquanZhu. 2013

[9]传染性法氏囊病病毒反向遗传技术发展及其应用DevelopmentandApplicationoftheReverseGeneticTechnologiesforInfectiousBursalDiseaseVirus. 祁小乐1,王永强1,高立1,高宏雷1,高玉龙1,王笑梅1,2*QIXiaole1,WANGYongqiang1,GAOLi1,GAOHonglei1,GAOYulong1,WANGXiaomei1,2*. 2015

[10]运用反向遗传学培养产生高成长性的猪流感疫苗(英文). TaoYangMingLiuChunguoLiuYunZhangDafeiLiuHaoChenBiotechnologyAnimalInfluenzaLaboratoryoftheMinistryAgricultureandNationalKeyLaboratoryofVeterinary,HarbinVeterinaryResearchInstituteofCAAS,Harbin150001,China. 2007

[11]表达H3N8亚型马流感病毒重组禽痘病毒载体疫苗的研究. 戚亭,郭巍,王晓钧. 2015

[12]IFITM3 restricts porcine deltacoronavirus infection by targeting its Spike protein. Wu J.,Tang R.,Zhang X.,Gao M.,Guo L.,Zhang L.,Shi D.,Zhang X.,Shi H.,Song H.,Feng L.,Chen J.. 2024

[13]The ORF1ab of Feline Coronavirus Plays a Critical Role in Regulating the Innate Immune Response. 谷昊容,,夏楚乔,,康洪涛,,贾洪林. 2025

[14]猪流行性腹泻病毒感染猪小肠上皮细胞miRNA表达谱分析及验证. 张宸语,陈佳宁,温建新,刘光亮. 2018

[15]中国猪病毒性腹泻流行概况及其科学防控. 冯力,陈建飞,时洪艳,张鑫. 2015

[16]2013-2014年全国23个地区猪腹泻病毒的流行病学调查. 张贺伟,张华,孟庆森,夏铭崎,王鑫,王炜,宋妮,宁晨,赵建增,武华. 2015

[17]猪流行性腹泻病毒感染小肠上皮细胞后诱导的天然免疫应答. 刘平黄,符芳,潘巧,应兰,陈建飞,冯力. 2014

[18]病毒宏基因组学分析腹泻仔猪肠道病毒群落及变异PEDV的分离和鉴定. 单同领,童光志. 2013

[19]猪流行性腹泻病毒IgA抗体间接ELISA方法的建立. 刘烨,张家林,王潇博,石达,时洪艳,陈建飞,张鑫,冯力. 2019

[20]STAT3在猪流行性腹泻病毒感染的作用及机制初步探索. 杨丽君,张健,许嘉宇,张璐,冯力,陈洪岩,王玉娥. 2019

作者其他论文 更多>>