数字农科院2.0

Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster

文献类型： 外文期刊

作者： Kang, Houxiang;Yang, Yuhong;Zhang, Zhonghua;Zhang, Shengping;Mao, Zhenchuan;Cheng, Guohua;Gu, Xingfang;Huang, Sanwen;Xie, Bingyan;Weng, Yiqun;Kang, Houxiang;Yang, Yuhong;Zhang, Zhonghua;Zhang, Shengping;Mao, Zhenchuan;Cheng, Guohua;Gu, Xingfang;Huang, Sanwen;Xie, Bingyan

作者机构：

关键词： genomic DNA;simple sequence repeat marker;Indel01;Indel02;Indel03;genome sequence;bacterial artiWcial chromosome

期刊名称： THEORETICAL AND APPLIED GENETICS

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年卷期：

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收录情况： SCI

摘要： Scab, caused by Cladosporium cucumerinum, is an important disease of cucumber, Cucumis sativus. In this study, we conducted fine genetic mapping of the single dominant scab resistance gene, Ccu, with 148 F-9 recombinant inbred lines (RILs) and 1,944 F-2 plants derived from the resistant cucumber inbred line 9110Gt and the susceptible line 9930, whose draft genome sequence is now available. A framework linkage map was first constructed with simple sequence repeat markers placing Ccu into the terminal 670 kb region of cucumber Chromosome 2. The 9110Gt genome was sequenced at 5x genome coverage with the Solexa next-generation sequencing technology. Sequence analysis of the assembled 9110Gt contigs and the Ccu region of the 9930 genome identified three insertion/deletion (Indel) markers, Indel01, Indel02, and Indel03 that were closely linked with the Ccu locus. On the high-resolution map developed with the F-2 population, the two closest flanking markers, Indel01 and Indel02, were 0.14 and 0.15 cM away from the target gene Ccu, respectively, and the physical distance between the two markers was approximately 140 kb. Detailed annotation of the 180 kb region harboring the Ccu locus identified a cluster of six resistance gene analogs (RGAs) that belong to the nucleotide binding site (NBS) type R genes. Four RGAs were in the region delimited by markers Indel01 and Indel02, and thus were possible candidates of Ccu. Comparative DNA analysis of this cucumber Ccu gene region with a melon (C. melo) bacterial artificial chromosome (BAC) clone revealed a high degree of micro-synteny and conservation of the RGA tandem repeats in this region.

分类号： R394

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