数字农科院2.0

Identification of the minimal active fragment of the Cry1Ah toxin

文献类型： 外文期刊

作者： Zhou, Zishan;Song, Fuping;Shu, Changlong;Zhang, Jie;Huang, Dafang

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关键词： Bacillus thuringiensis;Cry1Ah;Toxicity;Minimal active fragment;Plutella xylostella

期刊名称： BIOTECHNOLOGY LETTERS

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收录情况： SCI

摘要： cry1Ah1, a novel holo-type gene cloned from Bacillus thuringiensis strain BT-8, encoded a protein exhibiting strong insecticidal activity against lepidopteran insects. To identify the minimal active fragment of the Cry1Ah toxin, 9 pairs of primers were designed to generate different PCR products. Seven PCR products were amplified by different primers using the cry1Ah1 gene as a template and cloned into a pET-21b vector. These positive clones were separately transformed into Escherichia coli. Insecticidal activity against 2nd-instar larvae of Plutella xylostella was performed using the leaf-dip bioassay: the minimal active fragment of the Cry1Ah toxin was located between amino acid residues 50I and 639E.

分类号： Q7

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