数字农科院2.0

Additional far-red light promotes adventitious rooting of double-root-cutting grafted watermelon seedlings

文献类型: 外文期刊

作者: Wu, Xue;Zhang, Xiaoyan;Wang, Yaya;Wu, Cuinan;Sun, Yudong;Zhang, Yi;Ji, Yongran;Bao, Encai;Xia, Liru;Bian, Zhonghua;Cao, Kai

作者机构:

关键词: Watermelon;Double-root-cutting grafting;Rootstock;Adventitious root regeneration;Auxin

期刊名称: HORTICULTURAL PLANT JOURNAL

ISSN: 2095-9885

年卷期: 2024 年 10 卷 6 期

页码:

收录情况: SCIE(2024版) ; ; CSCD(2023-2024年度) ; ; 科技核心(2024版) ; ; 农林核心(2020版)

摘要: Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting. To date, little information is available on how to enhance root regeneration of rootstock in grafted watermelon (Citrullus lanatus) seedlings. In this study, the effects of different light treatments on root regeneration were determined. This revealed that addition of far-red light (Fr) could significantly expedite root formation in the rootstock. Moreover, the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr, especially for auxin-response proteins (including CmIAA11, CmIAA17, and CmAUX28), Small auxinup RNA genes (including CmSAUR20 and CmSAUR50) and the auxin efflux transporter (CmPIN3). In addition, the expression of Phytochrome Interacting Factor (PIFs), such as CmPIF1, CmPIF3 and CmPIF7, was remarkably increased by Fr. These genes may act together to activate auxinrelated pathways under Fr treatment. Based on the results of HPLC-MS/MS analysis, the concentrations of different auxin-types in adventitious root were significantly influenced by Fr. Furthermore, the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment, which was determined by the acid magenta dyeing experiment. Therefore, all the results suggested that Fr could induce AR formation in rootstocks, which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes. The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings, which will provide a theoretical basis for the speeding development of industrialized seedlings

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