数字农科院2.0

A functional chromogen gene C from wild rice is involved in a diferent anthocyanin biosynthesis pathway in indica and japonica

文献类型： 外文期刊

作者： 乔卫华;;王艳艳;;许睿;;杨子怡;;孙妍;;苏龙;;张莉珍;;王君瑞;;黄婧芬;;郑晓明;;杨庆文

作者机构：

期刊名称： Theoretical and Applied Genetics

ISSN： 0040-5752

年卷期： 2021 年

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Accumulation of anthocyanin is a desirable trait to be selected in rice domestication, but the molecular mechanism of anthocyanin biosynthesis in rice remains largely unknown. In this study, a novel allele of chromogen gene C, OrC1, from Oryza rufpongon was cloned and identifed as a determinant regulator of anthocyanin biosynthesis. Although OrC1 functions in purple apiculus, leaf sheath and stigma in indica background, it only promotes purple apiculus in japonica. Transcriptome analysis revealed that OrC1 regulates favonoid biosynthesis pathway and activates a few bHLH and WD40 genes of ternary MYB-bHLH-WD40 complex in indica. Diferentially expressed genes and metabolites were found in the indica and japonica backgrounds, indicating that OrC1 activated the anthocyanin biosynthetic genes OsCHI, OsF3H and OsANS and produced six metabolites independently. Artifcial selection and domestication of C1 gene in rice occurred on the coding region in the two subspecies independently. Our results reveal the regulatory system and domestication of C1, provide new insights into MYB transcript factor involved in anthocyanin biosynthesis, and show the potential of engineering anthocyanin biosynthesis in rice.

分类号：

  • 相关文献
作者其他论文 更多>>