数字农科院2.0

Mechanistic insights for efficient removal of intracellular and extracellular antibiotic resistance genes by iron-based nanocopper: Intracellular oxidative stress and internalization of nanocopper

文献类型： 外文期刊

作者： Wang, Hao;Yu, Ping;Guo, Xujing;Wang, Wenguo;Wang, Lan;Zhang, Hongwei;Deng, Liangwei;Yang, Hongnan;He, Ting;Wu, Peike;Zhang, Yunhong

作者机构：

关键词： Iron-based Nanocopper;Antibiotic resistance genes;Oxidative stress;Nanocopper internalization

期刊名称： JOURNAL OF HAZARDOUS MATERIALS

ISSN： 0304-3894

年卷期： 2025 年 484 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The widespread use of antibiotics has led to a severe pollution issue with antibiotic resistance genes (ARGs), which poses a significant threat to both ecological environments and human health. In this study, we developed an iron-based nanocopper bimetallic material (Fe-nCu) for the efficient removal of ARGs. Our results indicate that nCu can attach to the surface of iron, forming aggregated copper nanoclusters resembling wheat ears. The composition of Fe-nCu particles consists of 75.90 % iron and 20.95 % copper. Fe-nCu demonstrates a unique capability in eliminating ARGs, achieving removal efficiencies of 3.75 and 4.36 logs for intracellular and extracellular ARGs, respectively. Furthermore, Fe-nCu remains stable in complex water environments and is unaffected by organic substances in the water. This material induces oxidative stress in cells within a short period, leading to an imbalance in intracellular redox levels and resulting in cell membrane damage. nCu causes severe membrane damage to E. coli, penetrating the cell due to its size advantage, which leads to the encapsulation and internalization of E. coli by the copper nanoparticles. Once inside, the nCu particles cleave DNA and disrupt the function of ARGs. This study not only provides a cost-effective material for the removal of ARGs but also offers an in-depth understanding of the action mechanism of Fe-nCu, presenting a novel pathway for inhibiting the propagation of ARGs.

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