数字农科院2.0

Fine mapping and cloning of an intersubspecific reproductive isolation locus MSHB1 in rice

文献类型： 外文期刊

作者： Xuan Wang;Zhengjiu Zhang;Jinfeng Zhao;Mengjiao Chen;Junyi Gong;Yu Han;Xin Wei;Xuehui Huang;Jiongjiong Fan

作者机构：

关键词： Fine mapping;Genetic redundancy;Hybrid breakdown;Intersubspecific hybridization;Male sterility;Oryza sativa L

期刊名称： Molecular Breeding

ISSN： 1380-3743

年卷期： 2026 年 46 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Reproductive isolation between rice subspecies poses a significant barrier to harnessing heterosis in hybrid breeding. Here, we identified and characterized a novel locus, designated Male Sterility-Related Hybrid Breakdown Locus on Chromosome 1 (MSHB1), through the utilization of chromosome segment substitution lines (CSSLs) derived from crosses between an indica cultivar Huanghuazhan (HHZ) and an aromatic cultivar Basmati Surkh 89 − 15 (BAS). A near-isogenic line, CS004, carrying the BAS-derived MSHB1BAS allele, exhibits complete male sterility, reduced plant height, fewer panicles, and shorter panicle length. Fine mapping localized MSHB1 to a 28.583-kb interval on the short arm of chromosome 1. Genetic complementation confirmed that the MSHB1 allele from HHZ is sufficient to restore fertility. MSHB1 encodes a homolog of Esa1-associated factor 6 (EAF6), which regulates histone acetylation and transcriptional activity. Comparative genomic analyses revealed that functional divergence in promoter activity between duplicated OsEAF6 paralogs (MSHB1 and MSHB12) drives hybrid breakdown. These findings elucidate a molecular mechanism underlying intersubspecific reproductive isolation and highlight MSHB1 as a strategic target for overcoming hybrid sterility to enhance heterosis in rice breeding.

分类号：

  • 相关文献

[1]Fine mapping and candidate gene analysis of hwh1 and hwh2, a set of complementary genes controlling hybrid breakdown in rice. Jiang, Wenzhu,Chu, Sang-Ho,Piao, Rihua,Lee, Joohyun,Qiao, Yongli,Koh, Hee-Jong,Jiang, Wenzhu,Chu, Sang-Ho,Piao, Rihua,Lee, Joohyun,Qiao, Yongli,Koh, Hee-Jong,Chin, Joong-Hyoun,Jin, Yong-Mei,Jin, Yong-Mei,Han, Longzhi,Piao, Zongze.

[2]The molecular mechanisms of male reproductive organogenesis in rice (Oryza sativa L.). Tian, Yun,Yang, Hui,Dai, Qian,Fang, Jun,Lu, Xiang Yang,Tian, Yun,Yang, Hui,Dai, Qian,Fang, Jun,Lu, Xiang Yang,Tian, Yun,Qing, Xian Guo,Qing, Xian Guo,Zhang, Hai Wen,Zhang, Hai Wen.

[3]Fine mapping of the lesion mimic and early senescence 1 (lmes1) in rice (Oryza sativa). Li, Zhi,Zhang, Yingxin,Liu, Lin,Liu, Qunen,Bi, Zhenzhen,Yu, Ning,Cheng, Shihua,Cao, Liyong,Li, Zhi,Li, Zhi,Zhang, Yingxin,Cheng, Shihua,Cao, Liyong.

[4]A core collection and mini core collection of Oryza sativa L. in China. Zhang, Hongliang,Zhang, Dongling,Wang, Meixing,Sun, Junli,Qi, Yongwen,Li, Jinjie,Li, Zichao,Wei, Xinghua,Tang, Shengxiang,Han, Longzhi,Qiu, Zongen.

[5]Genomic insights on the contribution of introgressions from Xian/Indica to the genetic improvement of Geng/Japonica rice cultivars. Di Cui,Han Zhou,Xiaoding Ma,Zechuan Lin,Linhua Sun,Bing Han,Maomao Li,Jianchang Sun,Jin Liu,Guixiu Jin,Xianju Wang,Guilan Cao,Xing Wang Deng,Hang He,Longzhi Han. 2022

[6]Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding. Hu, Q,Hansen, LN,Laursen, J,Dixelius, C,Andersen, SB. 2002

[7]The Arabidopsis U-box/ARM repeat E3 ligase AtPUB4 influences growth and degeneration of tapetal cells, and its mutation leads to conditional male sterility. Wang, Hai,Jiang, Tiantian,Xia, Yiji,Wang, Hai,Berg, Howard,Xia, Yiji,Lu, Yuqing,Li, Cong. 2013

[8]Cysteine Protease 51 (CP51), an anther-specific cysteine protease gene, is essential for pollen exine formation in Arabidopsis. Yang, Yongxue,Dong, Caihua,Yu, Jingyin,Tong, Chaobo,Huang, Junyan,Liu, Shengyi,Yang, Yongxue,Shi, Lei,Li, Zhenbo.

[9]Identification of WA-type three-line hybrid rice with real-time Polymerase Chain Reaction (PCR) method. Cheng, Y.,Chen, H. Y.,Mao, J. J.,Cao, A. X.,Zhu, S. F.,Cheng, Y.,Gao, B. D.,Mao, J. J.,Zhu, J. G..

[10]A male sterile line with dominant gene (Ms) in cabbage (Brassica oleracea var. capitata) and its utilization for hybrid seed production. Fang, ZY,Sun, PT,Liu, YM,Yang, LM,Wang, XW,Hou, AF,Bian, CS. 1997

[11]Comparative sequence and expression analysis of tapetum specific male sterility related genes in Medicago truncatula. Shao, L. H.,Zheng, X. W.,Yi, D. X.,Li, C.. 2016

[12]Genetic and histological characterization of a novel recessive genic male sterile line of Brassica napus derived from a cross with Capsella bursa-pastoris. Chen, Hai-Feng,Ge, Xian-Hong,Du, Xue-Zhu,Zhao, Zhi-Gang,Li, Zai-Yun,Chen, Hai-Feng.

[13]Defective callose walls and cell plates during abnormal meiosis cause male-sterility in the oat mutant zbs1. Shi Xiao,Wu Jin-xia,Han Xiao,Zhou Hai-tao,Yang Xiao-hong,Li Tian-liang,Zhang Xin-jun,Yang Cai.

[14]Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis). Xu, Xiaoyong,Yang, Zeping,Sun, Xilu,Zhang, Lugang,Fang, Zhiyuan,Xu, Xiaoyong,Fang, Zhiyuan. 2014

[15]iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.). Ji Liu,Chaoyou Pang,Hengling Wei,Meizhen Song,Yanyan Meng,Jianhui Ma,Shuli Fan,Shuxun Yu.

[16]Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray. Bonnema, Guusje,Kang, Jungen,Zhang, Guoyu,Fang, Zhiyuan,Wang, Xiaowu.

[17]BoPMEI1, a pollen-specific pectin methylesterase inhibitor, has an essential role in pollen tube growth. Zhang, Guo Yu,Feng, Jing,Wu, Jian,Wang, Xiao Wu. 2010

[18]CRISPR/Cas9-targeted mutagenesis of TaDCL4, TaDCL5 and TaRDR6 induces male sterility in common wheat. Zhang, Rongzhi,Zhang, Shujuan,Li, Jihu,Gao, Jie,Song, Guoqi,Li, Wei,Geng, Shuaifeng,Liu, Cheng,Lin, Yanxiang,Li, Yulian,Li, Genying. 2023

[19]The Assessment of Natural Cross Pollination Properties of a Novel Male-Sterile–Female-Fertile Mutation msLC01 in Soybean. Wen Wang,Xiaojie Zhu,Yu Zhang,Huawei Gao,Zeru Zhang,Chunyan Yang,Yuhong Zheng,Qianqian Yu,Yixin Zhu,Yating Geng,Shulei Wang,Like Liu. 2023

[20]TRANSCRIPTOME ANALYSIS OF THE IMMATURE INFLORESCENCES OF FOXTAIL MILLET TREATED WITH SQ-1 FOR INDUCING MALE STERILITY. Wang, Chunfang,Qi, Dongmei,Zhang, Wei,Wang, Yongqiang,Shi, Shenkui,Diao, Xianmin. 2021

作者其他论文 更多>>