Novel liquid-phase blocking ELISA using single-domain antibody M8 and virus-like particles for in-vitro potency assessment of foot-and-mouth disease virus-like particle vaccine
文献类型: 外文期刊
作者: Haozhou Li;Aldo Dekker;Yaya Zhang;Yun Zhang;Michiel Harmsen;Wim van der Poel;Huichen Guo;Shiqi Sun
作者机构:
关键词: Foot-and-mouth disease;In vitro potency evaluation;Single domain antibody;Statistic model;Vaccine quality control;Virus like particle vaccine
期刊名称: Vaccine
ISSN: 0264-410X
年卷期: 2025 年 62 卷
页码:
收录情况: SCIE(2025版)
摘要: Potency tests for inactivated foot-and-mouth disease (FMD) vaccines involve either in vivo challenge trials in target animals or in vitro tests. However, evaluating potency of FMD virus-like particle (VLP) vaccines currently lack in vitro tools. We developed a liquid-phase blocking ELISA (LPBE) using a broadly neutralizing single-domain antibody (M8) and FMD VLPs to assess antibody titers in animals vaccinated with FMD serotype O VLPs. We utilized 505 negative sera and 32 sera of pigs infected or vaccinated with other viruses than FMDV to validate the M8 LPBE detection limit. The M8 LPBE was compared to virus neutralizing test and conventional LPBE using 338 sera from PD50 potency tests in cattle and pigs. The results were analyzed using logistic models. The M8 LPBE showed superior correlation with protection, with an average titer of 1.7 log10 corresponding to a potency of 3 PD50/dose.
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