数字农科院2.0

Manipulation of CBTS1 Expression Alters Tobacco Resistance to Spodoptera frugiperda and Phytophthora nicotianae

文献类型： 外文期刊

作者： Guan, Jian;Du, Zaifeng;Tian, Tian;Wang, Wenjing;Ju, Fuzhu;Lin, Xiaoyang;Zhang, Zhongfeng;Cao, Yi;Zhang, Hongbo

作者机构：

关键词： cembranoid;cembratrien-ol synthase;Spodoptera frugiperda;Phytophthora nicotianae;PR gene

期刊名称： AGRONOMY-BASEL

ISSN：

年卷期： 2023 年 13 卷 3 期

页码：

收录情况： SCIE（2023版）

摘要： Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 expression on tobacco resistance to the insect pest Spodoptera frugiperda and oomycete pathogen Phytophthora nicotianae. The CDS sequence of CBTS1 was cloned into gene overexpression and silencing vectors and introduced into tobacco (Nicotiana tabacum L. cv. TN90) to obtain CBTS1-overexpression plants (CBTS1-OE) and CBTS1-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The S. frugiperda resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to S. frugiperda was significantly improved but that of CBTS1-RI plants was reduced. The P. nicotianae resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to P. nicotianae was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of CBTS1 increased the expression of the pathogen-related gene PR-1a, while silencing of CBTS1 decreased its expression. This study demonstrated that manipulating the expression of CBTS1 could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens.

分类号：

  • 相关文献

[1]Investigating the spatiotemporal expression of CBTS genes lead to the discovery of tobacco root as a cembranoid-producing organ. Zaifeng Du,Tian Tian,Yulong Gao,Jian Guan,Fuzhu Ju,Shiquan Bian,Jiahao Wang,Xiaoyang Lin,Bingwu Wang,Zhihua Liao,Yongmei Du,Zhongfeng Zhang,Hongbo Zhang. 2024

[2]Integrated control of tobacco black shank by combined use of riboflavin and Bacillus subtilis strain Tpb55. Zhang, Chengsheng,Tian, Xueying,Wang, Fenglong,Gao, Jiaming,Han, Teng.

[3]Identification Of Stably Expressed Qtl F.or Resistance To Black S hank Disease In Tobacco (Nicotiana Tabacum L.) Line Beinhart 1000-1. Zhang, YS, Guo, X, Yan, XX, Ren, M, Jiang, CH, Cheng, YZ, Wen, LY, Liu, D, Zhang, Y, Sun, MM, Feng, QF, Yang, AG, Cheng, LR. 2018

[4]Genome analysis of phytophthora nicotianae jm01 provides insights into its pathogenicity mechanisms. Xiao Long Yuan,Cheng Sheng Zhang,Fan Yu Kong,Zhong Feng Zhang,Feng Long Wang. 2021

[5]Complete Mitochondrial Genome Of Phytophthora N.icotianae And Identification Of M olecular Markers For The Oomycetes. Yuan, XL, Feng, C, Zhang, ZF, Zhang, CS. 2017

[6]Extraction of honokiol from Artemisia argyi and in vitro and in vivo investigation of its antifungal activity. Wang, Xianxian,Han, Xiaobin,Wang, Shu,Wang, Yaobin,Wang, Peng,Zhao, Zhongli,Qin, Huimin,Jing, Changliang,Liang, Chen. 2022

[7]Rootstock-Scion Interaction Affects The Composition And Pathogen Inhibitory Activity Of Tobacco (Nicotiana Tabacum L.) Root Exudates. Zhang, CS, Zheng, YF, Peng, LJ, Cao, JM. 2020

[8]Honokiol inhibits growth and improves control efficiency against Phytophthora nicotianae through disrupting redox homeostasis and energy metabolism. Xianxian Wang,Yi Xie,Zhen Li,Qianru Chen,Jiali Sun,Xiaobin Han,Rui Liu,Zhiming Hu,Chen Liang,Chengsheng Zhang,Changliang Jing. 2022

[9]Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco. Sun, Mingming,Li, Lei,Wang, Chengdong,Wang, Luanming,Lu, Di,Shen, Danyu,Wang, Jie,Jiang, Caihong,Cheng, Lirui,Pan, Xuhao,Yang, Aiguo,Wang, Yuanying,Zhu, Xiaowei,Li, Bin,Li, Yiting,Zhang, Feng. 2022

[10]Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant And Susceptible Tobacco Cultivars Against Black Shank Disease. Zhang, CS, Feng, C, Zheng, YF, Wang, J, Wang, FL. 2020

[11]Chitooligosaccharides and Arbuscular Mycorrhizal fungi alleviate the damage by Phytophthora nicotianae to tobacco seedlings by inducing changes in rhizosphere microecology. Junqing Ma,Yang Li,Heng Zhou,Lixin Qi,Zhifan Zhang,Yanfen Zheng,Zheyan Yu,Zeeshan Muhammad,Xia Yang,Yi Xie,Qianru Chen,Ping Zou,Siqi Ma,Yiqiang Li,Changliang Jing. 2024

[12]Precise quantification of Phytophthora nicotianae in tobacco using digital PCR. Yuanyuan Liu,Jiali Li,Zining Guo,Tianci Shen,Song Lu,Xian Chen,Danmei Liu,Wei Wang,Yunhua Gao,Chao Feng,Di Wang. 2025

[13]Development and validation of a droplet digital PCR assay for sensitive detection and quantification of Phytophthora nicotianae. Yuanyuan Liu,Jiali Li,Zining Guo,Chao Feng,Yunhua Gao,Danmei Liu,Di Wang. 2025

[14]Phenotypic Performance Of Nine Genotypes O.f Cry1A.105/Cry2Ab2 Dual-Gene Resistant F all Armyworm On Non-Bt And Mon 89034 Maize. Niu, Ying,Niu, Ying,Price, Paula A.,Huang, Fangneng,Guo, Jianguo,Guo, Jianguo,Head, Graham P.. 2019

[15]Integrated Transcriptome and Metabolome Analysis to Identify Sugarcane Gene Defense against Fall Armyworm (Spodoptera frugiperda) Herbivory. Ao Mei Li,Miao Wang,Zhong Liang Chen,Cui Xian Qin,Fen Liao,Zhen Wu,Wei Zhong He,Prakash Lakshmanan,You Qiang Pan,Dong Liang Huang. 2022

[16]The invasive Spodoptera frugiperda (JE Smith) has displaced Ostrinia furnacalis (Guenee) as the dominant maize pest in the border area of southwestern China. Song, Yifei,Yang, Xianming,Li, Hui,Wu, Kongming. 2023

[17]Identification of an odorant receptor responding to sex pheromones in Spodoptera frugiperda extends the novel type-I PR lineage in moths. Zhang, Sai,Jacquin-Joly, Emmanuelle,Montagne, Nicolas,Liu, Fang,Liu, Yang,Wang, Guirong. 2023

[18]Characterization of five pigmentation genes as transgenic markers in Spodoptera frugiperda (Lepidoptera: Noctuidae). Ying Cheng,Peng Wang,Yuxiao Zeng,Wenwen An,Tao Wang,Yutao Xiao. 2023

[19]Fine Structure and Olfactory Reception of the Labial Palps of Spodoptera frugiperda. Qiuyan Chen,Xiaolan Liu,Song Cao,Baiwei Ma,Mengbo Guo,Jie Shen,Guirong Wang. 2021

[20]Sublethal Effects of the Microbial-Derived Insecticide Spinetoram on the Growth and Fecundity of the Fall Armyworm (Lepidoptera: Noctuidae). Zupeng Gao,Yu Chen,Kanglai He,Jingfei Guo,Zhenying Wang. 2021

作者其他论文 更多>>